Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds

  • Bioresour Technol. 2012 Jul:115:21-6. doi: 10.1016/j.biortech.2011.11.015.
Yuanyuan Liu  1 ,  Zhuotong Zeng ,  Guangming Zeng ,  Lin Tang ,  Ya Pang ,  Zhen Li ,  Can Liu ,  Xiaoxia Lei ,  Mengshi Wu ,  Pinyun Ren ,  Zhifeng Liu ,  Ming Chen ,  Gengxin Xie
Affiliations
  • 1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China.
PMID: 22137272 DOI: 10.1016/j.biortech.2011.11.015
Abstract

A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g(-1)), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity change between enzyme and substrate. Application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal.

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