Fluorescent dye cocktail for multiplex drug-site mapping on human serum albumin

Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel Fluorescent Dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a Fluorescent Dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.