Pharmacokinetics and tissue distribution study of camellianin A and its major metabolite in rats by liquid chromatography with tandem mass spectrometry

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Aug 1;997:200-9. doi: 10.1016/j.jchromb.2015.06.012.
Yunliang Zheng  1 ,  Xingjiang Hu  1 ,  You Zhai  2 ,  Jian Liu  1 ,  Guolan Wu  1 ,  Lihua Wu  1 ,  Jianzhong ShenTu  1
Affiliations
  • 1. Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China.
  • 2. Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China. Electronic address: [email protected].
PMID: 26117310 DOI: 10.1016/j.jchromb.2015.06.012
Abstract

Camellianin A is a major active constituent of Adinandra nitida. A LC-MS/MS method for the determination of camellianin A and its metabolite (camellianin B) in rat plasma and tissues was developed and applied to a pharmacokinetics and tissue distribution study. Samples were separated on a Waters HSS T3 column with a mobile phase consisted of methanol and water (containing 0.1% formic acid). MS/MS detection was carried out on a triple-quadruple mass spectrometer under negative ESI mode. Pharmacokinetics study showed that camellianin A was rapidly eliminated with a t1/2 of 92.6±41.4h and CL of 3.19±0.471L/min/kg. Additionally, camellianin A showed a low oral bioavailability of 2.99% and a narrow tissue distribution; however, camellianin B was proved to have a wide tissue distribution with brain penetration. The data presented in this study provides useful information for the further applications of A. nitida and camellianin A.

Keywords
Camellianin A; Camellianin B; HPLC–MS/MS; Pharmacokinetics; Tissue distribution.
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