Selective production of rubusoside from stevioside by using the sophorose activity of β-glucosidase from Streptomyces sp. GXT6

  • Appl Microbiol Biotechnol. 2015 Nov;99(22):9663-74. doi: 10.1007/s00253-015-6802-z.
Zilong Wang  1 ,  Jinpei Wang  1 ,  Minhua Jiang  1 ,  Yutuo Wei  1 ,  Hao Pang  2 ,  Hang Wei  1 ,  Ribo Huang  1  3 ,  Liqin Du  4
Affiliations
  • 1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China.
  • 2. National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi, 530007, China. [email protected].
  • 3. National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi, 530007, China.
  • 4. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530005, China. [email protected].
PMID: 26198882 DOI: 10.1007/s00253-015-6802-z
Abstract

In order to produce rubusoside, Enzymes with preferential specificity for the saccharide sophorose were tested for ability to produce rubusoside from stevioside. We identified BGL1, a β-glucosidase from Streptomyces sp. GXT6, as an enzyme for rubusoside production. Out of several saccharide substrates, BGL1 showed the most affinity to sophorose. This enzyme only hydrolyzes the glucose moiety of the sophoroside at C-13 in stevioside. Production of rubusoside was determined by (1)H and (13)C nuclear magnetic resonance (NMR). Thus, rubusoside was produced from stevioside and the stevioside conversion rate was 98.2 %. The production yield of rubusoside was 78.8 % in 6 h.

Keywords
Rubus suavissimus; Rubusoside; Sophorose; Stevioside; Streptomyces sp; β-Glucosidase.
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