Synthesis and preliminary antiproliferative activity of new pteridin-7(8H)-one derivatives

  • Eur J Med Chem. 2018 Jan 1:143:1396-1405. doi: 10.1016/j.ejmech.2017.10.037.
Zhong-Hua Li  1 Tao-Qian Zhao  1 Xue-Qi Liu  1 Bing Zhao  1 Chao Wang  1 Peng-Fei Geng  1 Ya-Quan Cao  1 Dong-Jun Fu  1 Li-Ping Jiang  2 Bin Yu  3 Hong-Min Liu  4
Affiliations
  • 1. Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Zhengzhou 450001, PR China; Key Laboratory of Technology of Drug Preparation (Zhengzhou University), Ministry of Education of China, Zhengzhou 450001, PR China; Key Laboratory of Henan Province for Drug Quality and Evaluation, Zhengzhou 450001, PR China; School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
  • 2. School of Basic Medical Science, Central South University, Changsha 410013, PR China.
  • 3. Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Zhengzhou 450001, PR China; Key Laboratory of Technology of Drug Preparation (Zhengzhou University), Ministry of Education of China, Zhengzhou 450001, PR China; Key Laboratory of Henan Province for Drug Quality and Evaluation, Zhengzhou 450001, PR China; School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: [email protected].
  • 4. Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Zhengzhou 450001, PR China; Key Laboratory of Technology of Drug Preparation (Zhengzhou University), Ministry of Education of China, Zhengzhou 450001, PR China; Key Laboratory of Henan Province for Drug Quality and Evaluation, Zhengzhou 450001, PR China; School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: [email protected].
Abstract

Pteridines are an important class of heterocyclic compounds with diverse biological activities. Here, we report a series of pteridin-7(8H)-one derivatives and their antiproliferative activities toward MKN-45, MGC-803, EC-109, and H1650. Structure-activity relationship studies showed that compound 12 exerted the most potent antiproliferative activity against MKN-45 and MGC-803 with the IC50 values of 4.32 and 7.01 μM, respectively. Besides, compound 12 induced morphological changes and Apoptosis of MKN-45 cells, increased expression of Bax, down-regulated expression of Bcl-2 and caused cleavage of Caspase-3/9. Additionally, we first reported the construction of the novel bicyclic 8,9-dihydro-7H-purine-8-carboxylate scaffold through the competitive 5-endo cyclization reaction with two C-N bonds and a chiral carbon center established.

Keywords
Antiproliferative activity; Apoptosis; Cell cycle arrest; Pteridin-7(8H)-one.