4-Hydroxycyclohexanecarboxylic Acid as a Substrate for Cyclohexanecarboxylic Acid Production during the " Zapatera " Spoilage of Spanish-Style Green Table Olives

Zapatera spoilage was reproduced in Spanish-style green olive brines adjusted to pH 5 and with a concentration of 5% (wt/vol) NaCl. A relationship between the formation of the compound responsible for the zapatera off odor, cyclohexanecarboxylic acid, and consumption of a new compound isolated from olive brines, 4-hydroxycyclohexanecarboxylic acid, was established. When the latter compound was added to a synthetic medium inoculated with a concentrated microbial suspension from a zapatera brine, cyclohexanecarboxylic acid was formed. 4-Hydroxy-cyclohexanecarboxylic acid was not detected in fresh olives, NaOH solutions, or water washes. In normal fermentation brines it was not detected after 30 days of brining, its concentration increasing in a progressive manner thereafter during typical lactic acid fermentation of Spanish-style green olives.