Induction of secondary metabolite production by hygromycin B and identification of the 1233A biosynthetic gene cluster with a self-resistance gene

  • J Antibiot (Tokyo). 2020 Jul;73(7):475-479. doi: 10.1038/s41429-020-0295-4.
Sho Kato  1  2 ,  Takayuki Motoyama  3 ,  Masakazu Uramoto  1 ,  Toshihiko Nogawa  1 ,  Takashi Kamakura  4 ,  Hiroyuki Osada  5  6
Affiliations
  • 1. Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan.
  • 2. Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan.
  • 3. Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan. [email protected].
  • 4. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
  • 5. Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan. [email protected].
  • 6. Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan. [email protected].
PMID: 32139880 DOI: 10.1038/s41429-020-0295-4
Abstract

We found that the protein synthesis inhibitor hygromycin B induced the production of secondary metabolites, including lucilactaene, NG-391, fusarubin, 1233A, and 1233B, in the filamentous fungus, Fusarium sp. RK97-94. We identified the biosynthetic gene cluster for 1233A, an HMG-CoA synthase inhibitor. The biosynthetic gene cluster consisted of four genes, one of which was involved in conferring self-resistance to 1233A.

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