Two new pregnane alkaloids from Pachysandra terminalis Sieb. et Zucc

  • Nat Prod Res. 2021 Nov;35(21):3888-3894. doi: 10.1080/14786419.2020.1744143.
Yangyang Zhang  1 ,  Yuze Li  1  2 ,  Shiqi Huang  1 ,  Yuwen Cui  3 ,  Huawei Zhang  1 ,  Wenli Huang  1 ,  Chong Deng  1 ,  Wei Wang  1 ,  Xiaomei Song  1
Affiliations
  • 1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, Shaanxi Province Key Laboratory of New Drugs and Chinese, School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, China.
  • 2. The College of Life Sciences, Northwest University, Xi'an, China.
  • 3. Department of Pharmacy, Xi'an Medical University, Xi'an, China.
PMID: 32208773 DOI: 10.1080/14786419.2020.1744143
Abstract

Two new pregnane Alkaloids, (20S)-20α-cinnamoylamino-3β-dimethylamino-5-en-pregnane (1) and (20S)-20α-cinnamoylamino-3β-dimethylamino-pregnane (2), and four known Alkaloids (+)-(20S)-20-(dimethylamino)-3-(3'R-isopropyl)-lactam-5α-pregn-2-en-4-one (3), axillaridine A (4), pachysamine M (5) and 20α-dimethylamino-16β-hydroxy-3β-senecioylamino-pregn-5-ene (6) were obtained from the whole herb of Pachysandra terminalis Sieb. et Zucc. Their structures were determined by various spectral techniques and computed electronic circular dichroism (ECD) data. Compounds 1-4 were tested for cytotoxicity against three human tumor cell lines and a human umbilical vein endothelial cell (HUVEC) line. Compound 4 exhibited moderate cytotoxicity against MCF-7, U251 and A549 cells with IC50 values of 15.01 ± 0.47 μM, 20.13 ± 1.34 μM and 20.04 ± 1.16 μM, respectively; compounds 1-3 showed weak cytotoxic activity against three tumor cells.

Keywords
Pachysandra terminalis Sieb. et Zucc.; alkaloids; cytotoxic activity; structure identification.
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