Recombination Monophosphoryl Lipid A-Derived Vacosome for the Development of Preventive Cancer Vaccines

  • ACS Appl Mater Interfaces. 2020 Oct 7;12(40):44554-44562. doi: 10.1021/acsami.0c15057.
Ruoyu Cheng  1 ,  Flavia Fontana  1 ,  Junyuan Xiao  2 ,  Zehua Liu  1 ,  Patrícia Figueiredo  1 ,  Mohammad-Ali Shahbazi  1  3 ,  Shiqi Wang  1 ,  Jing Jin  2 ,  Giulia Torrieri  1 ,  Jouni T Hirvonen  1 ,  Hongbo Zhang  2  4 ,  Tongtong Chen  5 ,  Wenguo Cui  2 ,  Yong Lu  5 ,  Hélder A Santos  1  6
Affiliations
  • 1. Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.
  • 2. Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Second Road, 200025 Shanghai, PR China.
  • 3. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, 45139-56184 Zanjan, Iran.
  • 4. Department of Pharmaceutical Sciences Laboratory and Turku Center for Biotechnology, Åbo Akademi University, FI-20520 Turku, Finland.
  • 5. Radiology Department, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, 200025 Shanghai, PR China.
  • 6. Helsinki Insititute of Life Science, HiLIFE, University of Helsinki, FI-00014 Helsinki, Finland.
PMID: 32960566 DOI: 10.1021/acsami.0c15057
Abstract

Recently, there has been an increasing interest for utilizing the host immune system to fight against Cancer. Moreover, Cancer vaccines, which can stimulate the host immune system to respond to Cancer in the long term, are being investigated as a promising approach to induce tumor-specific immunity. In this work, we prepared an effective Cancer vaccine (denoted as "vacosome") by reconstructing the Cancer cell membrane, monophosphoryl lipid A as a Toll-like Receptor 4 agonist, and egg phosphatidylcholine. The vacosome triggered and enhanced bone marrow dendritic cell maturation as well as stimulated the antitumor response against breast Cancer 4T1 cells in vitro. Furthermore, an immune memory was established in BALB/c mice after three-time preimmunization with the vacosome. After that, the immunized mice showed inhibited tumor growth and prolonged survival period (longer than 50 days). Overall, our results demonstrate that the vacosome can be a potential candidate for clinical translation as a Cancer vaccine.

Keywords
cancer cell membrane; cancer immunotherapy; cancer vaccines; liposomes.
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