Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation

  • Vet Microbiol. 2021 Jan;252:108908. doi: 10.1016/j.vetmic.2020.108908.
Wenping Cui  1 ,  Jin Huang  2 ,  Xiangyun Niu  3 ,  Hongqi Shang  4 ,  Zhou Sha  5 ,  Yongqiang Miao  6 ,  Huan Wang  7 ,  Ruichang Chen  8 ,  Kai Wei  9 ,  Ruiliang Zhu  10
Affiliations
  • 1. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China. Electronic address: [email protected].
  • 2. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 3. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 4. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 5. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 6. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 7. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 8. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: [email protected].
  • 9. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China. Electronic address: [email protected].
  • 10. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China. Electronic address: [email protected].
PMID: 33254056 DOI: 10.1016/j.vetmic.2020.108908
Abstract

The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship. The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1. Under these conditions, extraction yield of total polysaccharide was 6.5 % ± 0.19 %. Total polysaccharide was then purified by DEAE-52 cellulose and Sephadex G-200 gel. Three fractions, PPP-1, PPP-2, and PPP-3, were identified with molecular weight of 463.70, 99.41, and 26.97 kDa, respectively. Three polysaccharide fractions were all composed of 10 Monosaccharides in different proportions. Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid. The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent. In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05). The Antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major Antiviral component. The structure-activity relationship showed that the Antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors. These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic Antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for Antiviral agent.

Keywords
Antiviral activity; Avian leukosis virus subgroup J; Pinus massoniana pollen; Structure activity relationship.
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