miRNA-222-3p enhances the proliferation and suppresses the apoptosis of acute myeloid leukemia cells by targeting Axin2 and modulating the Wnt/β-catenin pathway

  • Biochem Biophys Res Commun. 2022 Sep 10:620:83-91. doi: 10.1016/j.bbrc.2022.06.054.
Zhenyan Liu  1 ,  Liang Zhong  2 ,  Wenran Dan  1 ,  Xuan Chu  1 ,  Chen Liu  2 ,  Xu Luo  1 ,  Zhonghui Zhang  1 ,  Yang Lu  1 ,  Peng Wan  1 ,  Xiao Wang  1 ,  Beizhong Liu  3
Affiliations
  • 1. Central Laboratory of Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China.
  • 2. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.
  • 3. Central Laboratory of Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China; Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China. Electronic address: [email protected].
PMID: 35780585 DOI: 10.1016/j.bbrc.2022.06.054
Abstract

MicroRNA (miRNA)-222-3p is overexpressed in numerous Tumors, where it acts as an oncogene. Although miRNA-222 is highly expressed in Acute Myeloid Leukemia (AML), its functions and the mechanisms underlying these functions have not yet been fully elucidated. This study aimed to investigate the regulatory roles of miRNA-222-3p in AML and the molecular mechanisms underlying these roles. In this study, we observed that miRNA-222-3p increased the viability and suppressed the Apoptosis of AML cells. Axin2 was demonstrated to be a direct target of miRNA-222-3p, which when overexpressed, inhibited Axin2 expression and stimulated the Wnt/β-catenin pathway. In contrast, upregulation of Axin2 expression levels reduced the viability and enhanced the Apoptosis of AML cells. Moreover, it partially reversed the effects of the miRNA-222-3p mimic on the proliferation and Apoptosis of, and modulation of the Wnt/β-catenin pathway in, AML cells. Taken together, this study provides strong evidence that miRNA-222-3p can serve as a molecular target for AML treatment.

Keywords
Acute myeloid leukemia; Axin2; MicroRNA-222-3p; Wnt/β-catenin pathway.
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