Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

  • Nat Commun. 2022 Oct 10;13(1):5974. doi: 10.1038/s41467-022-33526-z.
Heewon Cho  #  1 ,  Haw-Young Kwon  #  2 ,  Amit Sharma  3 ,  Sun Hyeok Lee  1 ,  Xiao Liu  4 ,  Naoki Miyamoto  2 ,  Jong-Jin Kim  5 ,  Sin-Hyeog Im  3  6  7 ,  Nam-Young Kang  8 ,  Young-Tae Chang  9  10  11
Affiliations
  • 1. School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 2. Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea.
  • 3. Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 4. Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 5. Department of Biology, Sunchon National University, Sunchon, 57922, Republic of Korea.
  • 6. ImmunoBiome Inc., Pohang, 37666, Republic of Korea.
  • 7. Institute for Convergence Research and Education in Advanced Technology, Yonsei University, Seoul, 03722, Republic of Korea.
  • 8. Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
  • 9. School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea. [email protected].
  • 10. Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea. [email protected].
  • 11. Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea. [email protected].
  • # Contributed equally.
PMID: 36216803 DOI: 10.1038/s41467-022-33526-z
Abstract

Macrophages play crucial roles in protecting our bodies from Infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

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