Hsa_circ_0000520 Promotes Non-Small Cell Lung Cancer Progression through the miR-1258/AKT3 Axis

  • J Oncol. 2022 Dec 24:2022:3676685. doi: 10.1155/2022/3676685.
Lan Han  1 ,  Hanxue Yang  1 ,  Wei Wei  1 ,  Fen Hu  1 ,  Limei Yuan  1
Affiliations
  • 1. Department of Oncology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Sciences, Xiangyang 441021, Hubei, China.
PMID: 36593867 DOI: 10.1155/2022/3676685
Abstract

Background: There are several previous studies suggesting that circular RNAs (circRNAs) are involved in tumorigenesis of Non-Small Cell Lung Cancer (NSCLC). Nevertheless, the role of circRNA_0000520 (circ_0000520) in this disease has not yet been studied.

Methods: circ_0000520, MicroRNA (miR)-1258, and Akt serine/threonine kinase 3 (Akt3) mRNA expression levels were detected by qPCR. CCK-8, EdU, and Transwell assays were utilized to detect NSCLC cells' malignant biological behaviors. The targeted relationship between miR-1258 and Akt3 3'-UTR or circ_0000520 was verified through the dual-luciferase reporter gene assay. Western blotting was utilized to measure the Akt3 expression after circ_0000520 and miR-1258 were selectively regulated.

Results: circ_0000520 was upregulated in NSCLC. Highly expressed circ_0000520 is linked to the NSCLC patient's advanced TNM stage and lymph node metastasis. circ_0000520 overexpression facilitated NSCLC cell growth, migration, and invasion. miR-1258 was identified as the downstream target of circ_0000520. miR-1258 overexpression weakened the effect of circ_0000520 overexpression on NSCLC cells. miR-1258 targeted and inhibited Akt3. circ_0000520 positively regulated the Akt3 expression in NSCLC cells by sponging miR-1258.

Conclusion: circ_0000520 upregulates Akt3 by competitively binding with miR-1258 to facilitate NSCLC progression.

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