Soft agar colony formation assay to quantify mouse embryonic fibroblast transformation after Salmonella infection

  • STAR Protoc. 2023 Sep 15;4(3):102379. doi: 10.1016/j.xpro.2023.102379.
Virginie Stévenin  1 ,  Jacques Neefjes  2
Affiliations
  • 1. Department of Cell and Chemical Biology, Oncode Institute, Leiden University Medical Center (LUMC), Leiden 2333ZC, the Netherlands. Electronic address: [email protected].
  • 2. Department of Cell and Chemical Biology, Oncode Institute, Leiden University Medical Center (LUMC), Leiden 2333ZC, the Netherlands.
PMID: 37379220 DOI: 10.1016/j.xpro.2023.102379
Abstract

Links between Bacterial infections and Cancer are actively investigated. Cost-effective assays to quantify Bacterial oncogenic potential can shed new light on these links. Here, we present a soft agar colony formation assay to quantify mouse embryonic fibroblast transformation after Salmonella Typhimurium Infection. We describe how to infect and seed cells in soft agar for anchorage-independent growth, a hallmark of cell transformation. We further detail automated cell colony enumeration. This protocol is adaptable to Other bacteria or host cells. For complete details on the use and execution of this protocol, please refer to Van Elsland et al.1.

Keywords
Cancer; Cell Culture; Microbiology.