Protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications

  • STAR Protoc. 2023 Dec 15;4(4):102717. doi: 10.1016/j.xpro.2023.102717.
Patrick R Smith  1 ,  Angela Meyer  2 ,  Sarah Loerch  3 ,  Zachary T Campbell  4
Affiliations
  • 1. University of Wisconsin-Madison, Department of Anesthesiology, School of Medicine and Public Health, Madison, WI 53792, USA. Electronic address: [email protected].
  • 2. University of Wisconsin-Madison, Department of Anesthesiology, School of Medicine and Public Health, Madison, WI 53792, USA.
  • 3. University of California, Santa Cruz, Department of Chemistry and Biochemistry, Santa Cruz, CA 95064, USA.
  • 4. University of Wisconsin-Madison, Department of Anesthesiology, School of Medicine and Public Health, Madison, WI 53792, USA. Electronic address: [email protected].
PMID: 37967016 DOI: 10.1016/j.xpro.2023.102717
Abstract

Sensory neurons play pervasive roles throughout biology. In vitro studies to probe their functions hinge on the successful application of primary Cell Culture. Here, we present a protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications. We describe steps for extracting dorsal root ganglia, preparing cultures, maintaining them for days in vitro, and performing immunocytochemical labeling. We also include special considerations with respect to additional downstream applications. For complete details on the use and execution of this protocol, please refer to Smith et al. (2021).1.

Keywords
Cell culture; Cell isolation; Cell-based Assays; Neuroscience.