Isolation and structure determination of a new depsipeptide crocapeptin C from the myxobacterium Melittangium boletus

  • Nat Prod Res. 2024 Jan 25:1-7. doi: 10.1080/14786419.2024.2308719.
Chanaphat Thetsana  1 ,  Ryo Kobayashi  2 ,  Kanitta Manadee  2  3 ,  Shinya Kodani  1  2  4
Affiliations
  • 1. Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
  • 2. Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka, Japan.
  • 3. Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
  • 4. College of Agriculture, Academic Institute, Shizuoka University, Shizuoka, Japan.
PMID: 38270086 DOI: 10.1080/14786419.2024.2308719
Abstract

A new cyclic depsipeptide, crocapeptin C (1), containing 3-amino-6-hydroxy-2-piperidone (Ahp) was isolated from the methanol extract of the myxobacterium Melittangium boletus. The chemical structure of crocapeptin C (1) was determined through NMR and ESI-MS analysis. The stereochemistries of the constituent Amino acids in crocapeptin C (1) were determined using the advanced Marfey's method and ROESY spectrum data. Crocapeptin C (1) exhibited potent inhibitory activity against chymotrypsin with an IC50 value of 0.5 µM.

Keywords
Melittangium boletus; Peptide; protease inhibitor.
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