Penicillinase as a marker in enzyme-linked immunosorbent assays for steroid hormones

  • J Steroid Biochem. 1983 Jul;19(1B):419-21. doi: 10.1016/0022-4731(83)90197-8.
U M Joshi ,  H P Shah ,  G M Sankolli
PMID: 6310239 DOI: 10.1016/0022-4731(83)90197-8
Abstract

Using penicillinase (E.C. 3.5.2.6) as marker and microtitre ELISA plates for immunoadsorption, sensitive and specific assays for two steroid Hormones viz. testosterone in plasma and estrone-3-glucuronide in urine were developed. The entire assay can be performed in 4 h and requires simple laboratory equipment such as an incubator and a colorimeter. The enzyme labelled Steroids have a shelf life of more than 3 years.

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