Cloning and functional expression of a human parathyroid hormone receptor

  • Eur J Pharmacol. 1993 Jul 15;246(2):149-55. doi: 10.1016/0922-4106(93)90092-n.
H Schneider  1 ,  J H Feyen ,  K Seuwen ,  N R Movva
Affiliations
  • 1. Sandoz Pharma Ltd, Preclinical Research, Basel, Switzerland.
PMID: 8397094 DOI: 10.1016/0922-4106(93)90092-n
Abstract

We have cloned a human receptor for parathyroid hormone from a kidney complementary DNA library. The deduced sequence of 593 Amino acids shows high homology to the previously cloned receptors from opossum and rat. Expressed in COS-1 cells, the human receptor binds to parathyroid hormone-(1-38) with high affinity (PKD = 8.5) and is functionally coupled to Adenylate Cyclase (pEC50 = 9.4). At high concentrations of agonist, the receptor also activates phosphoinositide turnover.