R-Phycoerythrin ammonium sulfate precipitate  (Synonyms: R-PE ammonium sulfate precipitate)

R-Phycoerythrin (R-PE) (ammonium sulfate precipitate) is found in Heterosiphonia japonica. R-Phycoerythrin (ammonium sulfate precipitate) is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin (concentrated solution) can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin (ammonium sulfate precipitate) can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex/Em = 496/578 nm)^[1][2].

R-Phycoerythrin ammonium sulfate precipitate needs to be desalted and the solution changed to phosphate buffer before use.
R-Phycoerythrin (10-100 μM, 4 h) is cytotoxic to S180 in a concentration-dependent manner (58%)^[2].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Specific steps^[2]:
1. Cell treatment and staining
1.1 S180 and SMC7721 cancer cells were cultured in RPMI1640 medium containing 10% fetal bovine serum and 1% penicillin/streptomycin to the logarithmic growth phase (37°C, 5% CO₂, 95% air humidity).
1.2 The cells were seeded at a density of approximately 5 × 10⁵/mL in serum-free RPMI1640 medium and incubated with different concentrations of R-Phycoerythrin (10, 25, 50, 100 μM) for 4 hours.
2. Light treatment
2.1 Use 496 nm argon laser to excite R-Phycoerythrin, β subunit, γ subunit, and 514 nm to excite α subunit.
2.2 Change to RPMI1640 medium containing 10% serum and continue incubation for 18 hours.
2.3 Use MTT method to detect the survival rate of S180 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

11016-17-4

[R-Phycoerythrin (ammonium sulfate precipitate)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sun L, et al. Isolation, purification and characteristics of R-phycoerythrin from a marine macroalga Heterosiphonia japonica. Protein Expr Purif. 2009 Apr;64(2):146-54.  [Content Brief]

  [2]. Huang B, et al. The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT. Cancer Biother Radiopharm. 2002 Feb;17(1):35-42.  [Content Brief]