RB104
RB104 is a highly selective neutral endopeptidase (NEP) inhibitor that exhibits extremely high affinity for Aplysia californica apNEP (Kd=0.16-0.26 pM) and rat NEP (Kd=7.0-26.8 pM). RB104 effectively blocks apNEP-mediated cleavage of Leu-enkephalin and shows minimal cross-reactivity with other zinc peptidases. Furthermore, RB104 can be iodinated or radiolabeled with 125I to serve as a probe, and is widely used in inhibitor gel electrophoresis, binding assays, Western blotting, and autoradiographic tissue localization, enabling sensitive detection of nanogram-level NEP in crude extracts and tissue sections.
For research use only. We do not sell to patients.
- CAS No.: 145253-48-1
- Formula: C21H23IN2O6
- Molecular Weight:526.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
[125I]RB-104 (200 pM; 1 h) specifically binds to neutral endopeptidase on rabbit renal cortical VSMC, with binding reduced by ~35% after 72 h of 10 ng/mL EGF treatment and increased by ~20% after 72 h of 10 ng/mL TGF-β treatment[1].
RB104 (1 μM; 15 min) inhibits apNEP-mediated enkephalin degradation in Aplysia californica CNS plasma membranes by 87%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 145253-48-1
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Molecular Weight 526.33
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Formula C21H23IN2O6
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SMILES
O=C(O)C(CC(=O)NC(CC(=O)NO)CC=1C=CC=CC1)CC2=CC=C(O)C(I)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
Purity & Documentation
References
[2]. Zappulla JP, et al. Cloning and characterization of Aplysia neutral endopeptidase, a metallo-endopeptidase involved in the extracellular metabolism of neuropeptides in Aplysia californica. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1999 Jun 01;19(11):4280-92. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
RB104 Related Classifications
HY-165399 Related Classifications
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