15 Lipoxygenase 2 Protein, Human (sf9)

15-Lipoxygenase 2 (15-LOX2) is a non-heme iron-containing dioxygenase crucial for peroxidation of polyunsaturated fatty acids (PUFAs). It exhibits peroxidase activity on arachidonate and linoleate, producing bioactive lipid mediators. Oxygenating arachidonyl derivatives, it generates 15-HETE-G, a peroxisome proliferator-activated receptor alpha agonist. 15-LOX2 class-switches pro-inflammatory mediators, participates in DHA oxidations to produce specialized pro-resolving mediators, and may influence cell cycle progression, proliferation, cytokine secretion, and macrophage differentiation. Importantly, it shows substrate specificity by not converting arachidonic acid to (15S)-HPETE. 15 Lipoxygenase 2 Protein, Human (sf9) is the recombinant human-derived 15 Lipoxygenase 2 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of 15 Lipoxygenase 2 Protein, Human (sf9) is 676 a.a., with molecular weight of 62-66 kDa.

15-Lipoxygenase 2 (15-LOX2) is a non-heme iron-containing dioxygenase with a crucial role in catalyzing stereo-specific peroxidation of both free and esterified polyunsaturated fatty acids (PUFAs), generating a diverse array of bioactive lipid mediators. This enzyme exhibits peroxidase activity on arachidonate, producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE, and on linoleate, generating 13-hydroperoxyoctadecadienoate/13-HPODE. Moreover, it oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG), leading to the production of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G), which acts as a peroxisome proliferator-activated receptor alpha agonist. 15-LOX2 efficiently class-switches pro-inflammatory mediators into anti-inflammatory intermediates and participates in the sequential oxidations of docosahexaenoate (DHA) to generate specialized pro-resolving mediators (SPMs) like resolvin D5. Besides its role in lipid metabolism, 15-LOX2 may influence cell cycle progression, cell proliferation, cytokine secretion by macrophages, and macrophage differentiation into proatherogenic foam cells. Importantly, it does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE, highlighting its substrate specificity.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

Tag Free

O15296 (N-G&P, M1-I676)

247  [NCBI]

62-66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.