200x Protein Detergent Buffer A

Customer Review

Based on 1 Customer Validation

This 200x protein detergent buffer A is formulated with 10% (w/v) DDM and 2% (w/v) CHS. DDM (n-Dodecyl β-D-Maltoside, CAS: 69227-93-6): maltoside-based non-ionic detergent. CHS (Cholesteryl Hemisuccinate Tris Salt, CAS: 102601-49-0): ionizable anionic detergent for stabilizing large unilamellar vesicles. DDM/CHS micelles form a membrane-like environment to solubilize membrane proteins and maintain their structural and functional integrity. This 200x protein detergent buffer A is sterile-filtered, contains no other preservatives, and must be diluted to 1× working solution with assay buffer prior to use.

For research use only. We do not sell to patients.
  • Species: /
  • Source: /
  • Storage:
    Stored at 4°C for 3 months from date of receipt, protect from light, Avoid any freeze-thaw cycles. lf precipitation forms please filter to clarify before use..
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

This 200x protein detergent buffer A is formulated with 10% (w/v) DDM and 2% (w/v) CHS. DDM (n-Dodecyl β-D-Maltoside, CAS: 69227-93-6): maltoside-based non-ionic detergent. CHS (Cholesteryl Hemisuccinate Tris Salt, CAS: 102601-49-0): ionizable anionic detergent for stabilizing large unilamellar vesicles. DDM/CHS micelles form a membrane-like environment to solubilize membrane proteins and maintain their structural and functional integrity. This 200x protein detergent buffer A is sterile-filtered, contains no other preservatives, and must be diluted to 1× working solution with assay buffer prior to use.

Technical Parameters

  • Species /
  • Source /
  • Accession
  • Gene ID
  • Synonyms

    Detergent Buffer; DDM/CHS

  • Molecular Weight

    /

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 10%(w/v) DDM and 2% (w/v) CHS.
Note: Due to the cytotoxicity of DDM, this product is not suitable for cell-based assays.

Reconstitution

Please dilute to 1× before use. Do not remove the detergent buffer under any condition.

Storage & Stability

Stored at 4°C for 3 months from date of receipt, protect from light, Avoid any freeze-thaw cycles. lf precipitation forms please filter to clarify before use..

Shipping

Shipping with blue ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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