200x Protein Detergent Buffer A
Based on 1 Customer Validation
This 200x protein detergent buffer A is formulated with 10% (w/v) DDM and 2% (w/v) CHS. DDM (n-Dodecyl β-D-Maltoside, CAS: 69227-93-6): maltoside-based non-ionic detergent. CHS (Cholesteryl Hemisuccinate Tris Salt, CAS: 102601-49-0): ionizable anionic detergent for stabilizing large unilamellar vesicles. DDM/CHS micelles form a membrane-like environment to solubilize membrane proteins and maintain their structural and functional integrity. This 200x protein detergent buffer A is sterile-filtered, contains no other preservatives, and must be diluted to 1× working solution with assay buffer prior to use.
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Storage:Stored at 4°C for 3 months from date of receipt, protect from light, Avoid any freeze-thaw cycles. lf precipitation forms please filter to clarify before use..
Biological Activity
This 200x protein detergent buffer A is formulated with 10% (w/v) DDM and 2% (w/v) CHS. DDM (n-Dodecyl β-D-Maltoside, CAS: 69227-93-6): maltoside-based non-ionic detergent. CHS (Cholesteryl Hemisuccinate Tris Salt, CAS: 102601-49-0): ionizable anionic detergent for stabilizing large unilamellar vesicles. DDM/CHS micelles form a membrane-like environment to solubilize membrane proteins and maintain their structural and functional integrity. This 200x protein detergent buffer A is sterile-filtered, contains no other preservatives, and must be diluted to 1× working solution with assay buffer prior to use.
Technical Parameters
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Synonyms
Detergent Buffer; DDM/CHS
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Molecular Weight
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Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 10%(w/v) DDM and 2% (w/v) CHS.
Note: Due to the cytotoxicity of DDM, this product is not suitable for cell-based assays.
Please dilute to 1× before use. Do not remove the detergent buffer under any condition.
Stored at 4°C for 3 months from date of receipt, protect from light, Avoid any freeze-thaw cycles. lf precipitation forms please filter to clarify before use..
Shipping with blue ice.
Documentation
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Data Sheet (230 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)