2. Recombinant Proteins
  3. Others
  4. 200x Protein Detergent Buffer A

This 200x protein detergent buffer A is formulated with 10% (w/v) DDM and 2% (w/v) CHS. DDM (n-Dodecyl β-D-Maltoside, CAS: 69227-93-6): maltoside-based non-ionic detergent. CHS (Cholesteryl Hemisuccinate Tris Salt, CAS: 102601-49-0): ionizable anionic detergent for stabilizing large unilamellar vesicles. DDM/CHS micelles form a membrane-like environment to solubilize membrane proteins and maintain their structural and functional integrity. This 200x protein detergent buffer A is sterile-filtered, contains no other preservatives, and must be diluted to 1× working solution with assay buffer prior to use.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
5 mL In-stock
> 5 mL   Get quote  
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

This 200x protein detergent buffer A is formulated with 10% (w/v) DDM and 2% (w/v) CHS. DDM (n-Dodecyl β-D-Maltoside, CAS: 69227-93-6): maltoside-based non-ionic detergent. CHS (Cholesteryl Hemisuccinate Tris Salt, CAS: 102601-49-0): ionizable anionic detergent for stabilizing large unilamellar vesicles. DDM/CHS micelles form a membrane-like environment to solubilize membrane proteins and maintain their structural and functional integrity. This 200x protein detergent buffer A is sterile-filtered, contains no other preservatives, and must be diluted to 1× working solution with assay buffer prior to use.
Source

/
Accession

/
Gene ID

/
Synonyms
Detergent Buffer; DDM/CHS
Molecular Weight

/
Appearance

Solution
Formulation

Supplied as a 0.22 μm filtered solution of 10%(w/v) DDM and 2% (w/v) CHS.
Note: Due to the cytotoxicity of DDM, this product is not suitable for cell-based assays.
Reconstitution

Please dilute to 1× before use. Do not remove the detergent buffer under any condition.
Storage & Stability

Stored at 4℃ for 3 months from date of receipt, protect from light. Avoid any freeze-thaw cycles. If precipitation forms, please filter to clarify before use.
Shipping

Shipping with blue ice.

Documentation
SDS (251 KB)

COA (217 KB)

Handling Instructions (2659 KB)

200x Protein Detergent Buffer A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

200x Protein Detergent Buffer A

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
200x Protein Detergent Buffer A
Cat. No.:
HY-P7B001
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.