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A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His)

The A1BG/Alpha-1B-Glycoprotein protein forms a complex with CRISP3, indicating a potential functional association. This interaction prompts further exploration of its biological consequences and regulatory mechanisms. The precise roles and implications within cellular processes or physiological pathways require additional research for a comprehensive understanding. A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His) is the recombinant human-derived A1BG/Alpha-1B-Glycoprotein protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His)
  • Experimental Validation Results for A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His)
  • A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

The A1BG/Alpha-1B-Glycoprotein protein forms a complex with CRISP3, indicating a potential functional association. This interaction prompts further exploration of its biological consequences and regulatory mechanisms. The precise roles and implications within cellular processes or physiological pathways require additional research for a comprehensive understanding. A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His) is the recombinant human-derived A1BG/Alpha-1B-Glycoprotein protein, expressed by HEK293 , with C-His labeled tag.

Background

The A1BG/Alpha-1B-Glycoprotein protein engages in specific molecular interactions, notably forming a complex with CRISP3. This interaction suggests a potential functional association between A1BG and CRISP3, prompting further investigation into the biological consequences and regulatory mechanisms influenced by their mutual binding. The nature and significance of this protein-protein interaction remain areas of interest, warranting additional research to unveil the precise roles and implications within cellular processes or physiological pathways.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human CRISP 3 at 1 μg/mL (100μL/Well) can bind biotinylated Human A1BG. The ED50 for this effect is 99.79 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P04217-1 (A22-S495)

  • Gene ID
    1  [NCBI]
  • Molecular Construction
    • N-term
    • A1BG (A22-S495)
      Accession # P04217
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    A1BG; HYST2477; Alpha-1-B Glycoprotein; A1B; Epididymis Secretory Sperm Binding Protein Li 163pA; ABG; Alpha-1B-Glycoprotein; GAB; HEL-S-163pA

  • AA Sequence

    AIFYETQPSLWAESESLLKPLANVTLTCQAHLETPDFQLFKNGVAQEPVHLDSPAIKHQFLLTGDTQGRYRCRSGLSTGWTQLSKLLELTGPKSLPAPWLSMAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES

  • Predicted Molecular Mass

    53.4 kDa

  • Molecular Weight

    Approximately 75 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human CRISP 3 at 1 μg/mL (100μL/Well) can bind biotinylated Human A1BG. The ED50 for this effect is 99.79 ng/mL.

  • A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His)
  • Experimental Validation Results for A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His)
  • A1BG/Alpha-1B-Glycoprotein Protein, Human (HEK293, His) Product Image