AANAT Protein, Human (His-GST)
Based on 1 Customer Validation
AANAT Protein, Human (His-GST) is the recombinant human-derived AANAT, expressed by E. coli, with N-10*His, N-GST labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
AANAT Protein, Human (His-GST) is the recombinant human-derived AANAT, expressed by E. coli, with N-10*His, N-GST labeled tag.
Background
AANAT regulates the day/night rhythm of melatonin production in the pineal gland. It catalyzes the N-acetylation of serotonin to form N-acetylserotonin, the penultimate step in melatonin synthesis.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His;N-GST
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Accession
Q16613-1 (M1-C207)
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Gene ID15
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Protein Length
Full Length of Isoform-1
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Synonyms
AANAT; Arylalkylamine N-Acetyltransferase; Aralkylamine N-Acetyltransferase; Serotonin Acetylase; Serotonin N-Acetyltransferase; AA-NAT; SNAT; DSPS
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AA Sequence
MSTQSTHPLKPEAPRLPPGIPESPSCQRRHTLPASEFRCLTPEDAVSAFEIEREAFISVLGVCPLYLDEIRHFLTLCPELSLGWFEEGCLVAFIIGSLWDKERLMQESLTLHRSGGHIAHLHVLAVHRAFRQQGRGPILLWRYLHHLGSQPAVRRAALMCEDALVPFYERFSFHAVGPCAITVGSLTFMELHCSLRGHPFLRRNSGC
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Predicted Molecular Mass
51.8 kDa
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Molecular Weight
Approximately 52 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)