ACE2 Protein, Mouse (HEK293, His-Fc)

ACE2 Protein, a crucial carboxypeptidase in the renin-angiotensin system, regulates blood volume and vascular resistance, maintaining cardiovascular homeostasis. It converts angiotensin I to anti-hypertrophic angiotensin 1-9 and angiotensin II to vasodilatory angiotensin 1-7, countering vasoconstriction. ACE2 also regulates amino acid transport, interacting with SLC6A19, and modulates vasoactive peptides like neurotensin and kinetensin. Its multifaceted functions make ACE2 pivotal in cardiovascular and amino acid homeostasis. ACE2 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived ACE2 protein, expressed by HEK293, with C-hFc, C-His, C-8*His labeled tag. The total length of ACE2 Protein, Mouse (HEK293, His-Fc) is 723 a.a., with molecular weight of ~130 kDa.

ACE2, an essential counter-regulatory carboxypeptidase within the renin-angiotensin hormone system, serves as a critical regulator of blood volume and systemic vascular resistance, contributing to cardiovascular homeostasis. Its enzymatic activity includes the conversion of angiotensin I to angiotensin 1-9, a peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, known for its vasodilatory and anti-proliferative properties, thereby counteracting the vasoconstrictor actions of angiotensin II. ACE2 further plays a role in amino acid transport, acting as a binding partner for the amino acid transporter SLC6A19, regulating its cell surface trafficking and activity. Additionally, ACE2 contributes to the regulation of various vasoactive peptides, such as neurotensin, kinetensin, and des-Arg bradykinin, as well as other biological peptides like apelins, casomorphins, and dynorphin A. Its multifaceted functions position ACE2 as a pivotal player in cardiovascular and amino acid homeostasis.

Measured by its binding ability in a functional ELISA. Immobilized Mouse ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 149.1 ng/mL, corresponding to a specific activity is 6706.9 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 149.1 ng/mL, corresponding to a specific activity is 6706.9 Unit/mg.

Mouse

HEK293

C-hFc;C-His;C-8*His

Q8R0I0-1 (Q18-T740)

70008  [NCBI]

QSLTEENAKTFLNNFNQEAEDLSYQSSLASWNYNTNITEENAQKMSEAAAKWSAFYEEQSKTAQSFSLQEIQTPIIKRQLQALQQSGSSALSADKNKQLNTILNTMSTIYSTGKVCNPKNPQECLLLEPGLDEIMATSTDYNSRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYNDYGDYWRGDYEAEGADGYNYNRNQLIEDVERTFAEIKPLYEHLHAYVRRKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFAQKPNIDVTDAMMNQGWDAERIFQEAEKFFVSVGLPHMTQGFWANSMLTEPADGRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYARQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSDFQEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFRGEIPKEQWMKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKYNGSLHKCDISNSTEAGQKLLKMLSLGNSEPWTKALENVVGARNMDVKPLLNYFQPLFDWLKEQNRNSFVGWNTEWSPYADQSIKVRISLKSALGANAYEWTNNEMFLFRSSVAYAMRKYFSIIKNQTVPFLEEDVRVSDLKPRVSFYFFVTSPQNVSDVIPRSEVEDAIRMSRGRINDVFGLNDNSLEFLGIHPTLEPPYQPPVT

Approximately 130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 1 mM ZnCl₂, pH 7.5 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.