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Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST, solution)

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Aconitase 2 (ACO2) protein catalyzes the isomerization of citric acid to isocitrate through the intermediate aconitate, playing a crucial role in cellular metabolism. This enzyme activity is a key step in the tricarboxylic acid (TCA) cycle, a central pathway for energy production and generation of metabolic intermediates. Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST, solution) is the recombinant mouse-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Aconitase 2 (ACO2) protein catalyzes the isomerization of citric acid to isocitrate through the intermediate aconitate, playing a crucial role in cellular metabolism. This enzyme activity is a key step in the tricarboxylic acid (TCA) cycle, a central pathway for energy production and generation of metabolic intermediates. Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST, solution) is the recombinant mouse-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

Aconitase 2 (ACO2) is an enzyme that plays a crucial role in the tricarboxylic acid (TCA) cycle by catalyzing the isomerization of citrate to isocitrate via the intermediate cis-aconitate. This reversible reaction is essential for the efficient metabolism of carbohydrates and the production of energy in the form of NADH. ACO2 acts as a key regulator in coordinating the flow of carbon through the TCA cycle, contributing to the generation of reducing equivalents and precursors for various biosynthetic pathways. The isomerization of citrate to isocitrate, facilitated by ACO2, represents a fundamental step in cellular energy production and metabolic homeostasis.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Mouse
  • Source Sf9 insect cells
  • Tag N-10*His;N-GST
  • Accession

    Q99KI0 (Q28-Q780)

  • Gene ID
    11429  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His-GST
    • ACO2 (Q28-Q780)
      Accession # Q99KI0
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    ACO2; Aconitase; Aconitase 2; Epididymis Secretory Sperm Binding Protein Li 284; Aconitate Hydratase, Mitochondrial; HEL-S-284; Citrate Hydro-Lyase; ICRD; ACONM; OCA8; Aconitase 2, Mitochondrial; OPA9; Mitochondrial Aconitase

  • AA Sequence

    QRAKVAMSHFEPSEYIRYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRTDIANLAEEFKDHLVPDPGCQYDQVIEINLNELKPHINGPFTPDLAHPVADVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLKCVIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQQ

  • Predicted Molecular Mass

    110 kDa

  • Molecular Weight

    Approximately 100 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, 0.5 mM GSH, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST, solution) Product Image