Alkaline Phosphatase/ALPP Protein, Human (Biotinylated, HEK293, His-Avi)
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
P05187 (I23-D506)
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Gene ID250
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Molecular Construction
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N-term
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ALPP (I23-D506)
Accession # P05187 -
His-Avi
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C-term
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Protein Length
Full Length of Mature Protein
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Conjugation
Biotin
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Synonyms
ALPP; PLAP-1; Alkaline Phosphatase, Placental; Alkaline Phosphatase, Placental (Regan Isozyme); PLAP; Placental Alkaline Phosphatase; PALP; Alkaline Phosphomonoesterase; Alkaline Phosphatase, Placental Type; Glycerophosphatase; Alkaline Phosphatase Regan
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AA Sequence
IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD
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Predicted Molecular Mass
56.3 kDa
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Molecular Weight
Approximately 65-70 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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