Allergen Fel d 4 Protein, Cat (His-SUMO)
Allergen Fel d 4 Protein, Cat (His-SUMO) is a recombinant protein with a His-SUMO tag. Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals.
- Species: Cat
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Allergen Fel d 4 Protein, Cat (His-SUMO) is a recombinant protein with a His-SUMO tag. Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals[1].
Background
Fel d 4 is a lipocalin allergen produced by the cat, which binds IgE at relatively high frequency in cat-sensitive individuals. The allergen provides not only a means for investigating differences in the immune response to lipocalin allergens from that found for other mammalian species but also an important reagent for the diagnosis of cat allergy[1].
Technical Parameters
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Species Cat
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Source E. coli
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Tag N-His;N-SUMO
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Accession
Q5VFH6 (H16-E186)
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Gene ID493739 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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Feld4 (H16-E186)
Accession # Q5VFH6 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Fel d 4; Allergen Fel d 4
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AA Sequence
HEEENVVRSNIDISKISGEWYSILLASDVKEKIEENGSMRVFVEHIKALDNSSLSFVFHTKENGKCTEIFLVADKTKDGVYTVVYDGYNVFSIVETVYDEYILLHLLNFDKTRPFQLVEFYAREPDVSQKLKEKFVKYCQEHGIVNILDLTEVDRCLQARGSEVAQDSSVE
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Predicted Molecular Mass
35.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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