2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Aminoacylase-1 Protein, Human (sf9, His)

Aminoacylase-1 Protein, Human (sf9, His)

Cat. No.: HY-P75576
Handling Instructions

The Aminoacylase-1 protein plays a crucial role in catalyzing the hydrolysis of N-acetylated amino acids, leading to the production of acetate and free amino acids. Aminoacylase-1 Protein, Human (sf9, His) is the recombinant human-derived Aminoacylase-1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Aminoacylase-1 Protein, Human (sf9, His) is 408 a.a., with molecular weight of ~44 kDa.

For research use only. We do not sell to patients.

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Description

The Aminoacylase-1 protein plays a crucial role in catalyzing the hydrolysis of N-acetylated amino acids, leading to the production of acetate and free amino acids. Aminoacylase-1 Protein, Human (sf9, His) is the recombinant human-derived Aminoacylase-1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Aminoacylase-1 Protein, Human (sf9, His) is 408 a.a., with molecular weight of ~44 kDa.
Background

Aminoacylase-1 Protein, an enzyme, plays a crucial role in the hydrolysis of N-acylated amino acids. Dysregulation of Aminoacylase-1 Protein has been associated with metabolic disorders and neurological diseases. Targeting Aminoacylase-1 Protein may offer potential therapeutic interventions in these conditions by modulating amino acid metabolism, improving metabolic function, and managing neurological disorders.
Species

Human
Source

Sf9 insect cells
Tag

C-His
Accession

Q03154 (M1-S408)
Gene ID

95  [NCBI]
Molecular Construction
N-term
Aminoacylase-1 (M1-S408)
Accession # Q03154
His
C-term
Synonyms
Aminoacylase-1; ACY-1; N-acyl-L-amino-acid amidohydrolase; Acy1
Molecular Weight

Approximately 44 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Aminoacylase-1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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