AMSH-LP Protein, Human (His)
AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSH-LP Protein, Human (His) is the recombinant human-derived AMSHLP protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSH-LP Protein, Human (His) is the recombinant human-derived AMSHLP protein, expressed by E. coli , with N-6*His labeled tag.
AMSHLP, a zinc metalloprotease, exhibits a selective ability to cleave 'Lys-63'-linked polyubiquitin chains, without affecting 'Lys-48'-linked polyubiquitin chains. Notably, AMSHLP serves as a positive regulator of the TORC1 signaling pathway through its role in the 'Lys-63'-linked deubiquitination of SESN2. This deubiquitination activity impedes the interaction between SESN2 and the GATOR2 complex, thereby contributing to the inhibition of SESN2-mediated TORC1 regulation. These functions underscore AMSHLP's specific involvement in fine-tuning cellular signaling pathways, particularly those associated with TORC1, through precise modulation of 'Lys-63'-linked polyubiquitin chains.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q96FJ0 (D2-R436)
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Gene ID57559
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Molecular Construction
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N-term
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6*His
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AMSHLP (D2-R436)
Accession # Q96FJ0 -
C-term
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Protein Length
Partial
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Synonyms
STAMBPL1; Associated Molecule With The SH3 Domain Of STAM (AMSH) - Family Protein; STAM Binding Protein Like 1; Associated Molecule With The SH3 Domain Of STAM (AMSH) Like Protein; AMSH-LP; AMSH-Like Protease; BA399O19.2; FLJ31524; KIAA1373; STAM Binding
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AA Sequence
DQPFTVNSLKKLAAMPDHTDVSLSPEERVRALSKLGCNITISEDITPRRYFRSGVEMERMASVYLEEGNLENAFVLYNKFITLFVEKLPNHRDYQQCAVPEKQDIMKKLKEIAFPRTDELKNDLLKKYNVEYQEYLQSKNKYKAEILKKLEHQRLIEAERKRIAQMRQQQLESEQFLFFEDQLKKQELARGQMRSQQTSGLSEQIDGSALSCFSTHQNNSLLNVFADQPNKSDATNYASHSPPVNRALTPAATLSAVQNLVVEGLRCVVLPEDLCHKFLQLAESNTVRGIETCGILCGKLTHNEFTITHVIVPKQSAGPDYCDMENVEELFNVQDQHDLLTLGWIHTHPTQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHKDTGIFRLTNAGMLEVSACKKKGFHPHTKEPRLFSICKHVLVKDIKIIVLDLR
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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