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Amyloid Precursor/Beta-APP40 Protein, Human (His-GST)

Cat. No.: HY-P75511
Handling Instructions

There is no specific Pubmed ID mentioned in the paragraph. Amyloid Precursor/Beta-APP40 Protein, Human (His-GST) is the recombinant human-derived Amyloid Precursor/Beta-APP40 protein, expressed by E. coli , with N-His, N-GST labeled tag. The total length of Amyloid Precursor/Beta-APP40 Protein, Human (His-GST) is 40 a.a., with molecular weight of ~33 kDa.

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Description

There is no specific Pubmed ID mentioned in the paragraph. Amyloid Precursor/Beta-APP40 Protein, Human (His-GST) is the recombinant human-derived Amyloid Precursor/Beta-APP40 protein, expressed by E. coli , with N-His, N-GST labeled tag. The total length of Amyloid Precursor/Beta-APP40 Protein, Human (His-GST) is 40 a.a., with molecular weight of ~33 kDa.

Background

APP (Amyloid Precursor Protein), also known as Protease Nexin-II, operates as a multifunctional cell surface receptor, exerting physiological effects on neurons that are crucial for neurite growth, neuronal adhesion, and axonogenesis. Its involvement in synaptogenesis is highlighted by the promotion of synaptic connections through interactions between APP molecules on adjacent cells. Beyond cell adhesion, APP plays a role in cell mobility and transcriptional regulation through protein-protein interactions. It can stimulate transcription activation by binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb. Additionally, APP couples to apoptosis-inducing pathways, such as those mediated by G(o) and JIP, and inhibits G(o) alpha ATPase activity. Acting as a kinesin I membrane receptor, APP facilitates axonal transport of beta-secretase and presenilin 1, contributing to axonal anterograde cargo transport towards synapses. In the context of copper homeostasis, APP is involved in copper ion reduction and can induce neuronal death through copper-metallated interactions. Furthermore, APP regulates neurite outgrowth by binding to extracellular matrix components and possesses protease inhibitor activity through its BPTI domain-containing isoforms. The protein participates in the AGER-dependent pathway, activating p38 MAPK and inducing internalization of amyloid-beta peptide, leading to mitochondrial dysfunction. Additionally, APP provides Cu(2+) ions for GPC1, required for nitric oxide release and heparan sulfate degradation. It exhibits metal-chelating properties, reduces transient metals, and binds to lipoproteins, apolipoproteins, and HDL particles, thereby modulating metal-catalyzed oxidation. APP's intricate involvement in various cellular processes underscores its significance in both normal neuronal function and pathological conditions associated with neurodegenerative disorders.

Species

Human

Source

E. coli

Tag

N-His;N-GST

Accession

P05067 (D672-V711)

Gene ID

351  [NCBI]

Molecular Construction
N-term
His-GST
APP (D672-V711)
Accession # P05067
C-term
Synonyms
Amyloid-beta precursor protein; APP; Protease Nexin II; PreA4
Molecular Weight

Approximately 33 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Amyloid Precursor/Beta-APP40 Protein, Human (His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Amyloid Precursor/Beta-APP40 Protein, Human (His-GST)
Cat. No.:
HY-P75511
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