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Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution)

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Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution), a recombinant human Arylsulfatase A produced in HEK293 cells, has a His tag. Arylsulfatase A is an important metabolic factor in pathophysiology of different diseases.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution)
  • Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution), a recombinant human Arylsulfatase A produced in HEK293 cells, has a His tag. Arylsulfatase A is an important metabolic factor in pathophysiology of different diseases[1].

Background

Arylsulfatase A is an enzyme that breaks down sulfatides. Arylsulfatase A has been demonstrated in preparations of human leukocytes. The level of activity of arylsulfatase A is markedly decreased in the preparations from patients with metachromatic leukodystrophy[1].

Verified Bioactivity

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS) that incubate at 37°C for 60min. The specific activity is 246.629 pmol/min/µg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P15289 (R19-A507)

  • Gene ID
    410  [NCBI]
  • Molecular Construction
    • N-term
    • ARSA (R19-A507)
      Accession # P15289
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    ARSA; Epididymis Secretory Sperm Binding Protein; Arylsulfatase A; Metachromatic Leucodystrophy; ASA; Arylsulfatase A Isoform 2; Cerebroside-Sulfatase; MLD

  • AA Sequence

    RPPNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGMYPGVLVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPATPCDGGCDQGLVPIPLLANLSVEAQPPWLPGLEARYMAFAHDLMADAQRQDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTADNGPETMRMSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPGVTHELASSLDLLPTLAALAGAPLPNVTLDGFDLSPLLLGTGKSPRQSLFFYPSYPDEVRGVFAVRTGKYKAHFFTQGSAHSDTTADPACHASSSLTAHEPPLLYDLSKDPGENYNLLGGVAGATPEVLQALKQLQLLKAQLDAAVTFGPSQVARGEDPALQICCHPGCTPRPACCHCPDPHA

  • Predicted Molecular Mass

    52.9 kDa

  • Molecular Weight

    Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. A K Percy, R O Brady. Metachromatic leukodystrophy: diagnosis with samples of venous blood. Science. 1968 Aug 9;161(3841):594-5. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution) Product Image
    1/16
  • Experimental Validation Results for Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution)
  • Arylsulfatase A/ARSA Protein, Human (HEK293, His, solution) Product Image