ASGR1/ASGPR1 Protein, Human (HEK293, hFc)
ASGR1/ASGPR1 mediates endocytosis of desialylated glycoproteins, recognizing galactose and N-acetylgalactosamine units. Ligand binding leads to internalization and sorting organelle transport, followed by receptor return to the cell membrane. ASGR1/ASGPR1 interacts with LASS2. ASGR1/ASGPR1 Protein, Human (HEK293, hFc) is the recombinant human-derived ASGR1/ASGPR1 protein, expressed by HEK293, with N-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ASGR1/ASGPR1 mediates endocytosis of desialylated glycoproteins, recognizing galactose and N-acetylgalactosamine units. Ligand binding leads to internalization and sorting organelle transport, followed by receptor return to the cell membrane. ASGR1/ASGPR1 interacts with LASS2. ASGR1/ASGPR1 Protein, Human (HEK293, hFc) is the recombinant human-derived ASGR1/ASGPR1 protein, expressed by HEK293, with N-hFc labeled tag.
Background
The ASGR1/ASGPR1 protein functions as a mediator in the endocytosis of plasma glycoproteins, particularly those from which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. This receptor demonstrates recognition capabilities for terminal galactose and N-acetylgalactosamine units. Upon ligand binding to the receptor, the formed complex undergoes internalization and is transported to a sorting organelle where the receptor and ligand disassociate. Subsequently, the receptor returns to the cell membrane surface. ASGR1/ASGPR1 engages in interactions with LASS2.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-hFc
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Accession
P07306-1 (Q62-L291)
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Gene ID432
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Molecular Construction
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N-term
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hFc
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ASGR1 (Q62-L291)
Accession # P07306 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
ASGR1; ASGR1 Protein; Asialoglycoprotein Receptor 1; ASGP-R 1; CLEC4H1; ASGPR 1; C-Type Lectin Domain Family 4 Member H1; ASGPR1; Hepatic Lectin H1; ASGPR; HL-1
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AA Sequence
QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
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Predicted Molecular Mass
52.0 kDa
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Molecular Weight
Approximately 55-70 kDa,based on Bis-Tris PAGE under reducing conditions,due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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