ATP2A2 Protein, Human (His)
Based on 1 Customer Validation
The ATP2A2 protein is a magnesium-dependent enzyme that hydrolyzes ATP and transfers calcium from the cytoplasm to the sarcoplasmic reticulum. It participates in autophagy during starvation and interacts with VMP1 to achieve ER isolating membrane contact. ATP2A2 Protein, Human (His) is the recombinant human-derived ATP2A2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The ATP2A2 protein is a magnesium-dependent enzyme that hydrolyzes ATP and transfers calcium from the cytoplasm to the sarcoplasmic reticulum. It participates in autophagy during starvation and interacts with VMP1 to achieve ER isolating membrane contact. ATP2A2 Protein, Human (His) is the recombinant human-derived ATP2A2 protein, expressed by E. coli , with N-6*His labeled tag.
The ATP2A2 protein, a magnesium-dependent enzyme, catalyzes the hydrolysis of ATP, facilitating the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Beyond its role in calcium transport, ATP2A2 is implicated in autophagy response to starvation, where it interacts with VMP1 to control ER-isolation membrane contacts for autophagosome formation. Additionally, ATP2A2 modulates ER contacts with lipid droplets, mitochondria, and endosomes, showcasing its versatile involvement in cellular processes. In collaboration with FLVCR2, ATP2A2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis. It also serves as a crucial regulator of TNFSF11-mediated Ca(2+) signaling pathways, interacting with TMEM64 to activate CREB1 and induce mitochondrial ROS generation. This intricate network of interactions underscores ATP2A2's pivotal role in the regulation of cellular processes, ranging from calcium homeostasis to autophagy and osteoclast differentiation.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P16615 (V314-M756)
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Molecular Construction
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N-term
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6*His
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ATP2A2 (V314-M756)
Accession # P16615 -
C-term
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Protein Length
Cytoplasmic Domain
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Synonyms
ATP2A2; ATPase Ca++ Transporting Cardiac Muscle Slow Twitch 2 Isoform 2; Prev. ATP2B; ATPase Ca++ Transporting Cardiac Muscle Slow Twitch 2; Prev. DAR; ATPase, Ca++ Dependent, Slow-Twitch, Cardiac Muscle-2; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPas
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AA Sequence
VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELNPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNM
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Predicted Molecular Mass
52.3 kDa
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Molecular Weight
Approximately 56-60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm sterile filtered PBS, 6% Trehalose, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
3.Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 100 mM NaCl, 6% Trehalose, pH 7.5.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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