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B3GAT1 Protein, Human (HEK293, His)

B3GAT1 Protein is pivotal in L2/HNK-1 carbohydrate epitope biosynthesis on glycoproteins and glycosaminoglycan biosynthesis.Catalyzing substrates like ASOR and asialo-fetuin, its enzymatic function depends on sphingomyelin presence, with stearoyl-sphingomyelin being the most effective.Notably, it exclusively acts on saturated fatty acids in sphingomyelin, showing no reactivity with unsaturated fatty acids, regardless of acyl group length.B3GAT1 Protein, Human (HEK293, His) is the recombinant human-derived B3GAT1 protein, expressed by HEK293 , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • B3GAT1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

B3GAT1 Protein is pivotal in L2/HNK-1 carbohydrate epitope biosynthesis on glycoproteins and glycosaminoglycan biosynthesis.Catalyzing substrates like ASOR and asialo-fetuin, its enzymatic function depends on sphingomyelin presence, with stearoyl-sphingomyelin being the most effective.Notably, it exclusively acts on saturated fatty acids in sphingomyelin, showing no reactivity with unsaturated fatty acids, regardless of acyl group length.B3GAT1 Protein, Human (HEK293, His) is the recombinant human-derived B3GAT1 protein, expressed by HEK293 , with N-6*His labeled tag.

Background

The B3GAT1 Protein plays a key role in the biosynthesis of the L2/HNK-1 carbohydrate epitope on glycoproteins and is implicated in glycosaminoglycan biosynthesis. The substrates involved in its catalytic activity encompass asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Intriguingly, the protein's enzymatic function is contingent upon the presence of sphingomyelin, with stearoyl-sphingomyelin proving to be the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Notably, the protein demonstrates activity exclusively with sphingomyelin harboring a saturated fatty acid, showing no reactivity with unsaturated fatty acids, irrespective of acyl group length.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-6*His
  • Accession

    Q9P2W7 (H25-I334)

  • Gene ID
    27087  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • B3GAT1 (H25-I334)
      Accession # Q9P2W7
    • C-term
  • Protein Length

    Lumenal Domain

  • Synonyms

    B3GAT1; GLCATP; Prev. CD57; LEU7 Antigen; Prev. LEU7; GlcUAT-P; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1; GLCUATP; Glucuronosyltransferase P; HNK1; GlcAT-P; NK-1; UDP-GlcUA:Glycoprotein Beta-1,3-Glucuronyltransferase; NK1; Beta-

  • AA Sequence

    HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

  • Predicted Molecular Mass

    36.2 kDa

  • Molecular Weight

    Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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