B7-H2/ICOSLG Protein, Rat (HEK293, His)

B7-H2/ICOSLG Protein, a ligand for T-cell receptor ICOS, critically costimulates T-cell proliferation and cytokine secretion. It induces B-cell proliferation and supports plasma cell differentiation, playing a key role in local tissue responses to inflammation. B7-H2/ICOSLG also modulates the secondary immune response by co-stimulating memory T-cell function. In molecular interactions, it has been observed to interact with CTLA4 in vitro. B7-H2/ICOSLG Protein, Rat (HEK293, His) is the recombinant rat-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-His labeled tag.

B7-H2/ICOSLG protein serves as a ligand for the T-cell-specific cell surface receptor ICOS, functioning as a critical costimulatory signal for T-cell proliferation and cytokine secretion. Additionally, it induces B-cell proliferation and facilitates their differentiation into plasma cells. This protein is poised to play a significant role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. In molecular interactions, B7-H2/ICOSLG has been shown to interact with CTLA4 in vitro.

Measured by its binding ability in a functional ELISA. Immobilized Rat B7-H2 at 1μg/mL (100 μL/well) can bind Mouse ICOS. The ED₅₀ for this effect is 89.92 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Rat B7-H2 at 1 μg/mL (100 μL/well) can bind Mouse ICOS. The ED₅₀ for this effect is 89.92 ng/mL

Rat

HEK293

C-His

XP_006223893 (E25-K261)

499415  [NCBI]

Partial

EVKEVNAMVGSDVELRCVYPRRSHFSLDDLYVYWQIVDEAKTVVTYYLPSANESSTIHVSNSYKNRAHLSPDLMKEGDFSLHLQNVTPQDTQEFKCLVFRMSTVLGKALEEVVRLRVAANFSTPVISTSGSSDPGQERTFTCMSKNGYPEPNLYWINRTDNTLIDETLQNNTVYLNELGLYDVVSTLRIPWTPHVDVICCVENVALHQNITSISRADSFTGSMNTERPQEIHREATK

Approximately 40-60 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.