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  3. Stimulatory Immune Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. B7-H2/ICOSLG B7-H2/CD275
  5. B7-H2/ICOSLG Protein, Rat (HEK293, His)

B7-H2/ICOSLG Protein, Rat (HEK293, His)

Cat. No.: HY-P75486
COA Handling Instructions

B7-H2/ICOSLG Protein, a ligand for T-cell receptor ICOS, critically costimulates T-cell proliferation and cytokine secretion. It induces B-cell proliferation and supports plasma cell differentiation, playing a key role in local tissue responses to inflammation. B7-H2/ICOSLG also modulates the secondary immune response by co-stimulating memory T-cell function. In molecular interactions, it has been observed to interact with CTLA4 in vitro. B7-H2/ICOSLG Protein, Rat (HEK293, His) is the recombinant rat-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

B7-H2/ICOSLG Protein, a ligand for T-cell receptor ICOS, critically costimulates T-cell proliferation and cytokine secretion. It induces B-cell proliferation and supports plasma cell differentiation, playing a key role in local tissue responses to inflammation. B7-H2/ICOSLG also modulates the secondary immune response by co-stimulating memory T-cell function. In molecular interactions, it has been observed to interact with CTLA4 in vitro. B7-H2/ICOSLG Protein, Rat (HEK293, His) is the recombinant rat-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-His labeled tag.

Background

B7-H2/ICOSLG protein serves as a ligand for the T-cell-specific cell surface receptor ICOS, functioning as a critical costimulatory signal for T-cell proliferation and cytokine secretion. Additionally, it induces B-cell proliferation and facilitates their differentiation into plasma cells. This protein is poised to play a significant role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. In molecular interactions, B7-H2/ICOSLG has been shown to interact with CTLA4 in vitro.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Rat B7-H2 at 1μg/mL (100 μL/well) can bind Mouse ICOS. The ED50 for this effect is 89.92 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Rat B7-H2 at 1μg/mL (100 μL/well) can bind Mouse ICOS. The ED50 for this effect is 89.92 ng/mL
Species

Rat

Source

HEK293

Tag

C-His

Accession

XP_006223893 (E25-K261)

Gene ID
Molecular Construction
N-term
B7-H2 (E25-K261)
Accession # XP_006223893
His
C-term
Synonyms
ICOS ligand; B7-H2; ICOSLG; LICOS; B7RP-1; CD275
AA Sequence

EVKEVNAMVGSDVELRCVYPRRSHFSLDDLYVYWQIVDEAKTVVTYYLPSANESSTIHVSNSYKNRAHLSPDLMKEGDFSLHLQNVTPQDTQEFKCLVFRMSTVLGKALEEVVRLRVAANFSTPVISTSGSSDPGQERTFTCMSKNGYPEPNLYWINRTDNTLIDETLQNNTVYLNELGLYDVVSTLRIPWTPHVDVICCVENVALHQNITSISRADSFTGSMNTERPQEIHREATK

Molecular Weight

Approximately 40-60 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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B7-H2/ICOSLG Protein, Rat (HEK293, His)
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HY-P75486
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