BCKDK Protein, Human
Based on 1 Customer Validation
BCKDK Protein, Human is the recombinant human-derived BCKDK, expressed by E. coli, with tag free labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
BCKDK Protein, Human is the recombinant human-derived BCKDK, expressed by E. coli, with tag free labeled tag.
BCKDK is a serine/threonine-protein kinase that functions as a metabolic regulator by forming a kinase-phosphatase pair with PPM1K. It coordinates branched-chain amino acid (BCAA) catabolism with glucose and lipid metabolism through phosphorylation of two distinct targets: 1) the mitochondrial BCKDHA subunit of BCKDH complex (inactivating it via Ser-337 phosphorylation to disrupt substrate channeling), and 2) cytosolic ACLY (phosphorylating Ser-455 during feeding transitions to promote lipogenesis/gluconeogenesis via MLXIPL/ChREBP-mediated transcriptional regulation). Recognizes phosphosites with SxxE/D motif.
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
O14874-1 (S31-I412)
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Gene ID10295
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Protein Length
Full Length of Mature Protein
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Synonyms
Branched-chain alpha-ketoacid dehydrogenase kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; 2.7.11.4; BCKDK; Homo sapiens; Human; Kinase; Transferase; 2.7.11.1
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AA Sequence
STSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI
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Predicted Molecular Mass
43.5 kDa
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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