BCKDK Protein, Human

BCKDK Protein, Human is the recombinant human-derived BCKDK, expressed by E. coli, with tag free labeled tag.

BCKDK is a serine/threonine-protein kinase that functions as a metabolic regulator by forming a kinase-phosphatase pair with PPM1K. It coordinates branched-chain amino acid (BCAA) catabolism with glucose and lipid metabolism through phosphorylation of two distinct targets: 1) the mitochondrial BCKDHA subunit of BCKDH complex (inactivating it via Ser-337 phosphorylation to disrupt substrate channeling), and 2) cytosolic ACLY (phosphorylating Ser-455 during feeding transitions to promote lipogenesis/gluconeogenesis via MLXIPL/ChREBP-mediated transcriptional regulation). Recognizes phosphosites with SxxE/D motif.

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

O14874-1 (S31-I412)

10295

Full Length of Mature Protein

STSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

≥ 80%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.