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  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. BirA Protein, E. coli (His-MBP)

BirA Protein, E. coli (His-MBP)

Cat. No.: HY-P75475
COA Handling Instructions

BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli , with N-His, N-MBP labeled tag.

For research use only. We do not sell to patients.

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Description

BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli , with N-His, N-MBP labeled tag.

Background

WBP2 protein functions as a transcriptional coactivator for estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation, binding to ESR1-responsive promoters in the presence of estrogen. It is essential for the coactivation function of YAP1 on PGR activity and synergizes with WBP2 in enhancing PGR activity. Additionally, WBP2 modulates the expression of post-synaptic scaffolding proteins through the regulation of ESR1, ESR2, and PGR. WBP2 interacts with the WW domain of YAP1, WWP1, and WWP2, and also forms interactions with NEDD4. Moreover, it associates with ESR1 and UBE3A, contributing to its role as a transcriptional coactivator in hormone-responsive pathways.

Biological Activity

Measured by its ability to generate pyrophosphate from the biotinylation reaction. The pyrophosphate is subsequently hydrolyzed using Recombinant Yeast Inorganic Pyrophosphatase/PPA1 (HY-P79310) that incubated at room temperature for 15 min. The specific activity is 654.92 pmol/min/μg.

Species

E.coli

Source

E. coli

Tag

N-His;N-MBP

Accession

P06709 (M1-K321)

Gene ID

948469  [NCBI]

Molecular Construction
N-term
His-MBP
BirA (M1-K321)
Accession # P06709
C-term
Synonyms
Bifunctional ligase/repressor BirA; birA; bioR; dhbB
AA Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK

Molecular Weight

Approximately 64-77 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0 or PBS, pH 7.4, 8% trehalose, 8% mannitol and 0.02% Tween80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

BirA Protein, E. coli (His-MBP) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BirA Protein, E. coli (His-MBP)
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