birA Protein, E. coli (His)
Based on 1 Customer Validation
The birA protein has the dual role of biotin ligase and biotin operon repressor. In the presence of ATP, it activates biotin to form the BirA-biotinyl-5'-adenylate complex. birA Protein, E. coli (His) is the recombinant E. coli-derived birA protein, expressed by E. coli , with N-6*His labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The birA protein has the dual role of biotin ligase and biotin operon repressor. In the presence of ATP, it activates biotin to form the BirA-biotinyl-5'-adenylate complex. birA Protein, E. coli (His) is the recombinant E. coli-derived birA protein, expressed by E. coli , with N-6*His labeled tag.
The BirA protein serves a dual role as both a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin, leading to the formation of the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can function by transferring the biotinyl group to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, thereby facilitating its activity. Alternatively, holoBirA can bind to the biotin operator site and inhibit the transcription of the operon. Despite its characterization as an orphan receptor, the specific ligand or function of the GPR146 protein remains unidentified.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-6*His
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Accession
Q8X709 (M1-K321)
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Gene ID914965
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Molecular Construction
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N-term
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6*His
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birA (M1-K321)
Accession # Q8X709 -
C-term
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Protein Length
Full Length
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Synonyms
Biotin-[acetyl-CoA carboxylase] synthetase; Biotin--protein ligase; EC:6.3.4.15; Biotin--[acetyl-CoA-carboxylase] ligase; Biotin operon repressor; Bifunctional ligase/repressor BirA
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AA Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELKSGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
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Predicted Molecular Mass
36.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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