birA Protein, E. coli (His)

The birA protein has the dual role of biotin ligase and biotin operon repressor. In the presence of ATP, it activates biotin to form the BirA-biotinyl-5'-adenylate complex. birA Protein, E. coli (His) is the recombinant E. coli-derived birA protein, expressed by E. coli , with N-6*His labeled tag.

The BirA protein serves a dual role as both a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin, leading to the formation of the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can function by transferring the biotinyl group to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, thereby facilitating its activity. Alternatively, holoBirA can bind to the biotin operator site and inhibit the transcription of the operon. Despite its characterization as an orphan receptor, the specific ligand or function of the GPR146 protein remains unidentified.

E.coli

E. coli

N-6*His

Q8X709 (M1-K321)

914965

Full Length

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELKSGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK

predict band size: 36.6 KDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.