1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Bone Morphogenetic Proteins (BMPs)
  5. Bone Morphogenetic Protein 5
  6. BMP-5 Protein, Human (HEK293, hFc)

BMP-5 Protein, Human (HEK293, hFc)

Cat. No.: HY-P75472
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Bone morphogenetic protein 5 (BMP-5) is a pleiotropic ligand protein belonging to the TGFβ family, which is mainly expressed in the lung and liver. After BMP-5 silencing, the activity of p38/ERK signaling pathway can be down-regulated to inhibit chondrocyte senescence and apoptosis and knee arthritis (OA). Bmp-5 initiates typical BMP signaling cascades by binding to type I receptor BMPR1A and type II receptor BMPR2, or triggers signals through atypical pathways such as MAPK p38 signaling cascades to promote chondrogenic differentiation. The total length of human BMP-5 protein is 454 amino acids (M1-H454), with 4 glycosylation domains. BMP-5 Protein, Human (HEK293, hFc) has a total length of 131 amino acids (Q324-H454), is expressed in HEK293 cells with a N-terminal hFc-tag.

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Description

Bone morphogenetic protein 5 (BMP-5) is a pleiotropic ligand protein belonging to the TGFβ family, which is mainly expressed in the lung and liver. After BMP-5 silencing, the activity of p38/ERK signaling pathway can be down-regulated to inhibit chondrocyte senescence and apoptosis and knee arthritis (OA)[1]. Bmp-5 initiates typical BMP signaling cascades by binding to type I receptor BMPR1A and type II receptor BMPR2[2], or triggers signals through atypical pathways such as MAPK p38 signaling cascades to promote chondrogenic differentiation[3]. The total length of human BMP-5 protein is 454 amino acids (M1-H454), with 4 glycosylation domains. BMP-5 Protein, Human (HEK293, hFc) has a total length of 131 amino acids (Q324-H454), is expressed in HEK293 cells with a N-terminal hFc-tag.

Background

Bone Morphogenetic Protein 5 (BMP-5) is a ligand protein with pleiotropic, belongs to TGFβ family and expresses mainly in the lung and liver. BMP/TGFβ signaling to involve in vascular and valvular homeostasis, which is a critical process of embryonic development[4]. And BMP/TGFβ signaling can be terminated by inhibitory SMADs including SMAD6 and SMAD7, which are activated and induced by BMP signaling and switch off BMP signaling via multiple mechanisms[5].
BMP-5 silencing inhibits chondrocyte senescence and apoptosis as well as osteoarthritis (OA) progression by downregulating activity in the p38/ERK signaling pathway[1].
BMP-5 initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2[2].
BMP-5 also triggers signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation[3].
BMP-5 specific expressing in neural crest progenitor cells is sufficient to induce cell proliferation through the MEK-ERK-ID3 signaling cascade, whereas disruption of this signaling cascade had no effect on cell survival[6].

In Vitro

BMP-5 (3 ng/mL; 1, 3, 5, 7, and 17 d) induces SCG neurons dendritic growth[2].
BMP-5 (1 ng/mL; , 3, and 6 min) induces phosphorylation of Smad-1 in cultured sympathetic neurons[2].
BMP-5 (.1-1 ng/mL; 6 d) is less potent than BMP-7 in dendritic growth in cultured sympathetic neurons, but equally efficacious in promoting dendritic growth in cultured sympathetic neurons[2].

Biological Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.7568 µg/mL, corresponding to a specific activity is 1.32×103 U/mg.

Species

Human

Source

HEK293

Tag

N-hFc

Accession

NP_066551.1 (Q324-H454)

Gene ID

653  [NCBI]

Molecular Construction
N-term
hFc
BMP-5 (Q324-H454)
Accession # NP_066551.1
C-term
Synonyms
Bone morphogenetic protein 5; BMP-5
Molecular Weight

50-55 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BMP-5 Protein, Human (HEK293, hFc)
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