C5AR1 Protein-VLP, Human (HEK293, His)
C5AR1 Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
C5AR1 Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
Background
The C5AR1 Protein-VLP serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand engages with at least two distinct sites on the receptor: a high-affinity site located on the extracellular N-terminus and a second site within the transmembrane region, initiating downstream signaling events. Upon receptor activation, a spectrum of cellular responses is triggered, including chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The receptor can exist as a homodimer and may form higher-order oligomers. When phosphorylated, C5AR1 interacts with ARRB1 and ARRB2, facilitating the internalization of C5aR. Additionally, the N-terminal domain of C5AR1 engages with S. aureus chemotaxis inhibitory protein (CHIPS), potentially inhibiting the immune response by blocking the receptor. This intricate molecular interplay underscores the multifaceted role of C5AR1 in orchestrating immune and inflammatory processes.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-10*His
-
Accession
P21730 (M1-V350)
-
Molecular Construction
-
N-term
-
C5AR1-VLP (M1-V350)
Accession # P21730 -
10*His
-
C-term
-
-
Protein Length
Full Length
-
Synonyms
C5AR1; C5a-R; Prev. C5R1; C5a Anaphylatoxin Chemotactic Receptor; C5AR; C5a Anaphylatoxin Receptor; CD88; CD88 Antigen; C5A; C5a Ligand; C5a Anaphylatoxin Chemotactic Receptor 1; C5aR; Complement Component 5a Receptor 1; Complement C5a Receptor 1; Complem
-
AA Sequence
MDSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLKKLDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV
-
Predicted Molecular Mass
41.1 kDa
-
Glycosylation
Yes
-
Purity
Purity analysis by SDS-PAGE is not available for VLP proteins.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
