Carbonic Anhydrase 9 Protein, Canine (HEK293, His)
Based on 1 Customer Validation
The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Canine (HEK293, His) is the recombinant canine-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Canine
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Canine (HEK293, His) is the recombinant canine-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag.
Background
Carbonic Anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion between carbon dioxide and water. It facilitates the formation of bicarbonate and hydrogen ions, the dissociated ions of carbonic acid. This enzymatic activity is essential for maintaining proper pH balance and regulating various physiological processes, including acid-base homeostasis, respiration, and ion transport. CA9 protein acts as a key player in the dynamic equilibrium of carbon dioxide and water, contributing to the efficient transport and regulation of these molecules in the body.
Verified Bioactivity
Measured by its esterase activity. The specific activity is 11.69 pmol/min/μg that incubate at room temperature in kinetic mode for 5 minutes.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Canine
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Source HEK293
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Tag C-6*His
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Accession
NP_001138646.1 (Q38-L410)
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Molecular Construction
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N-term
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CA9 (Q38-L410)
Accession # NP_001138646.1 -
His
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C-term
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Protein Length
Partial
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Synonyms
CA9; Carbonate Dehydratase IX; Carbonic Anhydrase 9; Carbonic Dehydratase; CAIX; Membrane Antigen MN; MN; P54/58N; Carbonic Anhydrase IX; CA-IX; Renal Cell Carcinoma-Associated Antigen G250; PMW1; RCC-Associated Protein G250; Carbonic Anhydrase; RCC-Assoc
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AA Sequence
QSLSRIQGTPGLGGDSSGEDDQLDEENLPSEEDPHGEEDPPGEEDPPGEEDPPGEEDPSGMKTEPGKEDSLKLEDLPTVEAPRDTPGSQNNAHRHNKGDDHSHWRYGGDPPWPQVSPACAGRFQSPVDIRPQLAALSPALPPLELLGFELPPLPELRLRNNGHTVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVDGHRFPAEIHVVHISTAFAKVEEALGRPGGLAVLAAFLQEGPEENSAYEQLLSHLEEITEEDSETWVPGLDVSALLPSDLSRYFRYEGSLTTPPCAQGVIWTVFNQTVRLSAKQLHTLSGSLWGPDDSRLQLNFRATQPLNGRIIEASFPAQVESSPRSFEPVHLNSCL
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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