1. Recombinant Proteins
  2. CAR-T Related Proteins Enzymes & Regulators Biotinylated Proteins
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 9 (CA IX)
  5. Carbonic Anhydrase 9 Protein, Human (Biotinylated, HEK293, Avi-His)

Carbonic Anhydrase 9 Protein, Human (Biotinylated, HEK293, Avi-His)

Cat. No.: HY-P72348
Handling Instructions Technical Support

The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

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사이즈 가격 재고 수량
20 μg 해외재고보유
100 μg 해외재고보유
> 100 μg   견적 받기  

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  • Biological Activity

  • Technical Parameters

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  • 제품 설명

제품 설명

The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

Background

Carbonic Anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion between carbon dioxide and water. It facilitates the formation of bicarbonate and hydrogen ions, the dissociated ions of carbonic acid. This enzymatic activity is essential for maintaining proper pH balance and regulating various physiological processes, including acid-base homeostasis, respiration, and ion transport. CA9 protein acts as a key player in the dynamic equilibrium of carbon dioxide and water, contributing to the efficient transport and regulation of these molecules in the body.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-Avi;C-6*His

Accession

Q16790 (Q38-D414)

Gene ID

768  [NCBI]

Molecular Construction
N-term
CA9 (Q38-D414)
Accession # Q16790
Avi-6*His
C-term
Protein Length

Extracellular Domain

Conjugation

Biotin

Synonyms
CA9; CA-IX; Carbonic Anhydrase IX; Carbonate dehydratase IX; G250; MN; P54/58N; RCC; RCC-associated protein G250
AA Sequence

QRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGD

분자량

Approximately 57 kDa

Glycosylation
Yes
Purity

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

선적

Room temperature in continental US; may vary elsewhere.

각종 서류

Carbonic Anhydrase 9 Protein, Human (Biotinylated, HEK293, Avi-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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상품명:
Carbonic Anhydrase 9 Protein, Human (Biotinylated, HEK293, Avi-His)
Cat. No.:
HY-P72348
수량:
MCE Japan Authorized Agent: