2. Protéines recombinantes
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Carboxylesterase Protein, Thermobifida fusca

Carboxylesterase Protein, Thermobifida fusca is the recombinant Carboxylesterase, expressed by E. coli , with tag Free labeled tag.

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Description

Carboxylesterase Protein, Thermobifida fusca is the recombinant Carboxylesterase, expressed by E. coli , with tag Free labeled tag.
Species

Others
Source

E. coli
Tag

Tag Free
Accession

P86325 (M1-L497)
Gene ID

/
Protein Length

Full Length
Synonyms
Carboxylesterase; Thermobifida fusca; Thermomonospora fusca; Hydrolase; Serine esterase; 3.1.1.1
AA Sequence

MEIVIRTGSGDVRGSKENGIAVFRGIPYAEPPVGAHRFTAPRPPRPWDGVRDATEFSATAPRPPYPEAIGALLIERFIPGDDYLTLNVWTPDPNAVGLPVMVWIHGGAFTNGSGSEPVYDGAAFARDGVVFVSFNYRLGIIGFADLPDAPSNRGLLDQIAALEWVRDNIARFGGDPGNVTVFGESAGAMSVCTLMATPRARGLFRRAILQSGAGNMAVAAEDATTIAAVIAHRLGVEPTAAALAHVPVAQLLDVQQQVAQEIQGAPDPAVWGERIAGGSVLLPFAPVIDGELLSQRPAEAIAGGAGHDVDLLFGTTTDEYRLFLAPTGLLPFITSDYVTAHLAKSGLDADAAKAYTAEGRGEEPGDILASIITDQVFRIPALRIAESRVDAPARTFGYEFAWRTPQLDGILGACHAVELPFVFRTLDRAASLVGTNPPEELAETVHNAWVRFATSGDPGWPAWNPETRSVMRFDHPVSEMVTDPYPATRALWDGVPL

Predicted Molecular Mass
53.1 kDa
Pureté

≥ 95%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Instruction de manipulation (2659 KB)

Carboxylesterase Protein, Thermobifida fusca Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Carboxylesterase Protein, Thermobifida fusca
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HY-P703494
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