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Catalase Protein, Human

3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

Catalase Protein, Human is an approximately 60.0 kDa human catalase protein expressed in E.coli. Catalase is a key enzyme in metabolism of H2O2 and reactive nitrogen species, and its expression and localization is markedly altered in tumors.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Catalase Protein, Human
  • Experimental Validation Results for Catalase Protein, Human
  • Catalase Protein, Human Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Top Publications Citing

Use of MCE Products

Biological Activity

Description

Catalase Protein, Human is an approximately 60.0 kDa human catalase protein expressed in E.coli. Catalase is a key enzyme in metabolism of H2O2 and reactive nitrogen species, and its expression and localization is markedly altered in tumors[1].

Background

Catalase is a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide[1][2].

Verified Bioactivity

Specific activity is 9471.979 units/mg. One unit will decompose 1.0 µmole of H2O2 per minute at pH 8.0 at 25 ℃.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P04040 (A2-L527)

  • Gene ID
    847  [NCBI]
  • Molecular Construction
    • N-term
    • Catalase (A2-L527)
      Accession # P04040
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CAT; Epididymis Secretory Sperm Binding Protein; Catalase

  • AA Sequence

    ADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITKYSKAKVFEHIGKKTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDPILFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNANGEAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL

  • Predicted Molecular Mass

    59.7 kDa

  • Molecular Weight

    Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filter solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Christophe Glorieux, et al. Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem. 2017 Sep 26;398(10):1095-1108. [Content Brief]

[2]. Diane E Heck, et al. Mechanisms of oxidant generation by catalase. Ann N Y Acad Sci. 2010 Aug;1203:120-5. [Content Brief]

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    Catalase Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):1999-2017.e10. [Abstract]

    Customer Validation
     The JNK-c-Jun activity is significantly stimulated by low-glucose culture and 2-Deoxy-D-glucose (2-DG) treatment, which can be antagonized by exogenous Catalase Protein, Human (catalase; 100 unit/mL; 2 h), in Cal27 cells.

  • Catalase Protein, Human Product Image
    1/16
  • Experimental Validation Results for Catalase Protein, Human
  • Experimental Validation Results for Catalase Protein, Human
  • Catalase Protein, Human Product Image

  • Catalase Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):1999-2017.e10. [Abstract]

    The JNK-c-Jun activity is significantly stimulated by low-glucose culture and 2-Deoxy-D-glucose (2-DG) treatment, which can be antagonized by exogenous Catalase Protein, Human (catalase; 100 unit/mL; 2 h), in Cal27 cells.