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RANTES/CCL5 Protein, Human

7 Cited Publications
Customer Review

Based on 7 publication(s) in Google Scholar

RANTES/CCL5 Protein is a secreted protein located outside the cell membrane and is a key pro-inflammatory chemokine and immune regulatory molecule in the CC chemokine family. RANTES/CCL5 Protein sends signals through its specific G protein-coupled receptors (GPCR) CCR1, CCR3, and CCR5, mediating inflammatory immune responses, viral infections, and tumor development. RANTES/CCL5 Protein can promote endothelial cell migration, proliferation, and angiogenesis in rats. RANTES/CCL5 Protein is useful for research into tumors, autoimmune diseases, and atherosclerosis. RANTES/CCL5 Protein, Human is a recombinant human CCL5 (S24-S91) protein expressed by E. coli.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • RANTES/CCL5 Protein, Human Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

RANTES/CCL5 Protein is a secreted protein located outside the cell membrane and is a key pro-inflammatory chemokine and immune regulatory molecule in the CC chemokine family. RANTES/CCL5 Protein sends signals through its specific G protein-coupled receptors (GPCR) CCR1, CCR3, and CCR5, mediating inflammatory immune responses, viral infections, and tumor development. RANTES/CCL5 Protein can promote endothelial cell migration, proliferation, and angiogenesis in rats. RANTES/CCL5 Protein is useful for research into tumors, autoimmune diseases, and atherosclerosis. RANTES/CCL5 Protein, Human is a recombinant human CCL5 (S24-S91) protein expressed by E. coli[1][2].

Background

CCL5, also known as RANTES (Regulated upon Activation, Normal T Cell Expressed and Secreted), is part of the CC subfamily of chemokines. The CCL5 gene is located in the q11.2-q12 region of human chromosome 17 and encodes a CCL5 protein with a molecular weight of 8 kDa[1].
RANTES/CCL5 Protein can be secreted by many cell types, such as platelets, endothelial cells, smooth muscle cells, macrophages, and activated T cells[1].
RANTES/CCL5 Protein can induce the expression of MMP-1 and MMP-13 in human rheumatoid arthritis synovial fibroblasts by utilizing heparan sulfate proteoglycans (HSPGs) and/or selectively activating PKCδ, JNK, and ERK proteins in the PKCδ-JNK/ERK pathway, ultimately leading to collagen degradation[3].
RANTES/CCL5 Protein promotes angiogenesis by binding to GPCR, CCR1, and CCR5, as well as to its proteoglycan receptors SDC-1, SDC-4, and CD-44, and it partially relies on VEGF secreted by endothelial cells[1].
RANTES/CCL5 Protein is induced by the relevant cytokine IL-13 in mice infected with RSV, leading to airway hyperreactivity (AHR) and worsening airway diseases[4].
RANTES/CCL5 Protein interacts with CCR5, increasing the phosphorylation of MEK and ERK in the MEK/ERK pathway, activating NF-κB, leading to the activation of αvβ3 integrin and promoting the migration of human osteosarcoma cells[5].
The oligomeric form of RANTES/CCL5 Protein is important for some of its functions. RANTES/CCL5 Protein can form larger aggregates in neutral pH environments, while it forms smaller aggregates when the pH is lowered[6].
RANTES/CCL5 Protein is associated with various pathological processes, including viral infections, atherosclerosis, inflammatory diseases, autoimmune diseases, and tumors[1].

In Vitro

RANTES/CCL5 Protein (1 or 10 nM, 25 days) induces angiogenesis in a rat intervertebral disc angiogenesis model[1].
RANTES/CCL5 Protein (100 ng/mL, 24 h) can activate the expression of MMP-1 and MMP-13 in human rheumatoid arthritis synovial fibroblasts, thereby inducing collagen degradation[3].
RANTES/CCL5 Protein (3 ng/mL, 24 h) can promote the expression of α v β 3 integrin and cell migration in human osteosarcoma cells[6].
Reduced mTOR activity in RANTES/CCL5 Protein expression-deficient Rantes KO stem/progenitor cells results in a younger haematopoietic stem cell phenotype[7].

In Vivo

RANTES/CCL5 Protein is induced to produce in primary respiratory syncytial virus infected mice, exacerbating airway diseases[4].
Lack of expression of RANTES/CCL5 Protein in Rantes gene knockout (KO) mice can lead to a decrease in myeloid biased HSCs and myeloid progenitor cells, as well as an increase in T cells and lymphoid biased HSCs[7].

Verified Bioactivity

1.Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10.0 ng/mL.
2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 2.640-4.751 ng/mL.
3. Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 3.0-4.0 ng/mL, corresponding to a specific activity is ≤ 3.004×105 U/mg.

Results
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
    Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 4.751 ng/mL, corresponding to a specific activity is 2.105×105 U/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P13501 (S24-S91)

  • Gene ID
    6352  [NCBI]
  • Molecular Construction
    • N-term
    • CCL5 (S24-S91)
      Accession # P13501
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    C-C Motif Chemokine 5; EoCP; Eosinophil Chemotactic Cytokine; SIS-Delta; Small-Inducible Cytokine A5; T Cell-Specific Protein P228; TCP228; T-Cell-Specific Protein RANTES; CCL5; D17S136E; SCYA5

  • AA Sequence

    SPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQVCANPEKKWVREYINSLEMS

  • Predicted Molecular Mass

    7.8 kDa

  • Molecular Weight

    Approximately 9-12 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for RANTES/CCL5 Protein, Human
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, 6% trehalose, 4% mannitol, 0.05% Tween 80, pH 4.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Suffee N, et al. RANTES/CCL5-induced pro-angiogenic effects depend on CCR1, CCR5 and glycosaminoglycans. Angiogenesis. 2012 Dec;15(4):727-44. [Content Brief]

[2]. Maillard L, et al. RANTES/CCL5 mediated-biological effects depend on the syndecan-4/PKCα signaling pathway. Biol Open. 2014 Sep 26;3(10):995-1004. [Content Brief]

[3]. Agere SA, et al. RANTES/CCL5 Induces Collagen Degradation by Activating MMP-1 and MMP-13 Expression in Human Rheumatoid Arthritis Synovial Fibroblasts. Front Immunol. 2017 Oct 18;8:1341. [Content Brief]

[4]. Tekkanat KK, et al. RANTES (CCL5) production during primary respiratory syncytial virus infection exacerbates airway disease. Eur J Immunol. 2002 Nov;32(11):3276-84. [Content Brief]

[5]. Wang SW, et al. CCL5 and CCR5 interaction promotes cell motility in human osteosarcoma. PLoS One. 2012;7(4):e35101. [Content Brief]

[6]. Wang X, et al. Oligomeric structure of the chemokine CCL5/RANTES from NMR, MS, and SAXS data. Structure. 2011 Aug 10;19(8):1138-48. [Content Brief]

[7]. Ergen AV, et al. Rantes/Ccl5 influences hematopoietic stem cell subtypes and causes myeloid skewing. Blood. 2012 Mar 15;119(11):2500-9. [Content Brief]

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Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 4.751 ng/mL, corresponding to a specific activity is 2.105×105 U/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 4.751 ng/mL, corresponding to a specific activity is 2.105×105 U/mg.

  • 1/16

    RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 21:e00950. [Abstract]

    Customer Validation
     RANTES/CCL5 Protein, Human (CCL5; 3,10, 30, 100, 300 ng/mL; 4 h) exhibited chemotactic effect on neutrophils when its concentration reached ng/mL. Freshly isolated neutrophils were suspended in serum-free medium and placed to the 3 µm upper chamber and the different medium were placed into the lower chamber. After 4 hours, the recruited neutrophils in lower chamber were collected and counted.

  • 1/16

    RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 21:e00950. [Abstract]

    Customer Validation
     Neutrophils freshly isolated from healthy donors were cultured in RPMI 1640 with or without LPS (5 µg/mL),RANTES/CCL5 Protein, Human (CCL5; 200 ng/mL) or CXCL10 (200 ng /mL) for 4 h, and stained with SYTOX Green (green) to evaluate Nets.

  • 1/16

    RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 May 20;13(5):478. [Abstract]

    Customer Validation
     RANTES/CCL5 Protein, Human (CCL5; 20, 50 ng/mL; 48 h) increased both migration and invasion abilities of Huh7 and Hep3B cells.

  • 1/16

    RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 May 20;13(5):478. [Abstract]

    Customer Validation
     The expression of EMT markers in Huh7 and Hep3B cells treated with RANTES/CCL5 Protein, Human (hCCL5; 20, 100 ng/ml; 48 h) was examined by western blotting.

  • 1/16

    RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 May 20;13(5):478. [Abstract]

    Customer Validation
     The expression of HIF1α in Huh7 and Hep3B cells treated with RANTES/CCL5 Protein, Human (hCCL5; 20, 100 ng/ml; 48 h) was detected by RT-PCR and western blotting.

  • RANTES/CCL5 Protein, Human Product Image
    1/16
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • Experimental Validation Results for RANTES/CCL5 Protein, Human
  • RANTES/CCL5 Protein, Human Product Image

  • RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 21:e00950. [Abstract]

    RANTES/CCL5 Protein, Human (CCL5; 3,10, 30, 100, 300 ng/mL; 4 h) exhibited chemotactic effect on neutrophils when its concentration reached ng/mL. Freshly isolated neutrophils were suspended in serum-free medium and placed to the 3 µm upper chamber and the different medium were placed into the lower chamber. After 4 hours, the recruited neutrophils in lower chamber were collected and counted.

  • RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 21:e00950. [Abstract]

    Neutrophils freshly isolated from healthy donors were cultured in RPMI 1640 with or without LPS (5 µg/mL),RANTES/CCL5 Protein, Human (CCL5; 200 ng/mL) or CXCL10 (200 ng /mL) for 4 h, and stained with SYTOX Green (green) to evaluate Nets.

  • RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 May 20;13(5):478. [Abstract]

    RANTES/CCL5 Protein, Human (CCL5; 20, 50 ng/mL; 48 h) increased both migration and invasion abilities of Huh7 and Hep3B cells.

  • RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 May 20;13(5):478. [Abstract]

    The expression of EMT markers in Huh7 and Hep3B cells treated with RANTES/CCL5 Protein, Human (hCCL5; 20, 100 ng/ml; 48 h) was examined by western blotting.

  • RANTES/CCL5 Protein, Human purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 May 20;13(5):478. [Abstract]

    The expression of HIF1α in Huh7 and Hep3B cells treated with RANTES/CCL5 Protein, Human (hCCL5; 20, 100 ng/ml; 48 h) was detected by RT-PCR and western blotting.