Copy Link Print Information

MCP-1/CCL2 Protein, Human

9 Cited Publications
Customer Review

Based on 9 publication(s) in Google Scholar

MCP-1/CCL2 Protein, Human is a cytokine belonging to the CC chemokine family that interacts with the CCR2 chemokine receptor on the cell surface to mediate inflammatory immune responses, viral infections, and tumorigenesis. MCP-1/CCL2 Protein MCP-1/CCL2 Protein, Human is a recombinant human MCP-1/CCL2(Q24-T99) expressed by E.coil.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MCP-1/CCL2 Protein, Human
  • Experimental Validation Results for MCP-1/CCL2 Protein, Human
  • Experimental Validation Results for MCP-1/CCL2 Protein, Human
  • MCP-1/CCL2 Protein, Human Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

MCP-1/CCL2 Protein, Human is a cytokine belonging to the CC chemokine family that interacts with the CCR2 chemokine receptor on the cell surface to mediate inflammatory immune responses, viral infections, and tumorigenesis. MCP-1/CCL2 Protein MCP-1/CCL2 Protein, Human is a recombinant human MCP-1/CCL2(Q24-T99) expressed by E.coil[1].

Background

CCL2, also known as monocyte chemotactic protein 1 (MCP1), is a small cell factor belonging to the CC chemokine family. The CCL2 gene, located in the q11.2-q12 region of human chromosome 17, encodes a monomeric polypeptide with a molecular weight of 9-15 kDa, depending on the level of glycosylation. CCL2 is mainly secreted by monocytes, macrophages and dendritic cells. It is secreted by monocytes, macrophages and dendritic cells, and platelet-derived growth factor is the main inducer of the CCL2 gene. Astrocytes and microglia are also thought to be the source of CCL2[1]. CCL2 signals through binding to and activation of CCR2 and induces a strong chemotactic response and intracellular mobilization of calcium ions. Among other things, CCL2/CCR2 can regulate cell adhesion and chemotaxis of macrophages by activating the β1 integrin and p38-MAPK signaling pathways. In addition to acting as a chemoattractant, CCL2 can also regulate brain endothelial permeability in vitro by altering tight junction (TJ) proteins and regulating the expression of endothelial adhesion molecules and leukocyte integrins as well as cytokine production. In addition, the CCL2-CCR2 signaling axis has been implicated in many inflammatory and neurodegenerative diseases, acting to recruit inflammatory cells into the CNS[2].  Originally described as a "tumor-derived chemokine", CCL2 has been shown to be a potent chemokine for many types of immune cells and a potential target for the treatment of many diseases, such as atherosclerosis, multiple sclerosis, asthma, neuropathic pain, diabetic nephropathy, and cancer[3].

In Vitro

CCL2 (10 ng/mL, 24 h) is secreted from tumor-associated macrophages (TAMs) co-cultured with MCF10A human breast epithelial cells, induces ERO1-α, leading to MMP-9 upregulation and an invasive phenotype of MCF10A cells[4].
              CCL2 (1-100 ng/mL, 96 h) can induce PC-3 and VCaP cell migration, proliferation, and Akt phosphorylation in a dose-dependent manner. In addition, CCL2 stimulates phosphorylation of p70-S6 kinase (a downstream target of Akt) and induces actin rearrangement[5].

Verified Bioactivity

1.The ED50 is <1 μg/mL as measured by CHO-K1/Gα15/hCCR2 cells (human Gα15 and human CCR2 stably expressed in CHO-K1 cells).
2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 this effect is 10-50 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P13500 (Q24-T99)

  • Gene ID
    6347  [NCBI]
  • Molecular Construction
    • N-term
    • CCL2 (Q24-T99)
      Accession # P13500
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CCL2; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2; Prev. SCYA2; Chemokine (C-C Motif) Ligand 2; MCP-1; Monocyte Chemotactic Protein 1; MCP1; Small-Inducible Cytokine A2; MCAF; C-C Motif Chemokine 2; HC11; MGC9434; Monocyte Chemotactic And Act

  • AA Sequence

    QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

  • Predicted Molecular Mass

    8.7 kDa

  • Molecular Weight

    Approximately 9-13 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Qiongyu Hao, et al.  CCL2/CCR2 signaling in cancer pathogenesis. Cell Commun Signal. 2020 May 29;18(1):82. [Content Brief]

[2]. Svetlana M Stamatovic, et al. Monocyte chemoattractant protein-1 regulation of blood-brain barrier permeability. J Cereb Blood Flow Metab. 2005 May;25(5):593-606. [Content Brief]

[3]. Rachel N Gomes, et al. Bacterial clearance in septic mice is modulated by MCP-1/CCL2 and nitric oxide. Shock. 2013 Jan;39(1):63-9. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 this effect is 48.89 ng/mL, corresponding to a specific activity is 2.05×104 U/mg.

  • 1/16

    MCP-1/CCL2 Protein, Human purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Jul 7;6(7):e70207. [Abstract]

    Customer Validation
     Inhibitory effects of Wj1113 on MCP‐1‐induced THP‐1 chemotaxis. THP‐1 cells were pretreated with different concentrations of Wj1113 for 48 h, then seeded into transwell chambers, and 250 ng/mL MCP-1/CCL2 Protein, Human (MCP‐1) in the lower chamber was used to stimulate for 3.5 h.

  • MCP-1/CCL2 Protein, Human Product Image
    1/16
  • Experimental Validation Results for MCP-1/CCL2 Protein, Human
  • Experimental Validation Results for MCP-1/CCL2 Protein, Human
  • Experimental Validation Results for MCP-1/CCL2 Protein, Human
  • MCP-1/CCL2 Protein, Human Product Image

  • MCP-1/CCL2 Protein, Human purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Jul 7;6(7):e70207. [Abstract]

    Inhibitory effects of Wj1113 on MCP‐1‐induced THP‐1 chemotaxis. THP‐1 cells were pretreated with different concentrations of Wj1113 for 48 h, then seeded into transwell chambers, and 250 ng/mL MCP-1/CCL2 Protein, Human (MCP‐1) in the lower chamber was used to stimulate for 3.5 h.