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CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi)

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CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein, thereby limiting inflammation by inhibiting TNF-α, interferons, and iNOS. CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD200R1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein, thereby limiting inflammation by inhibiting TNF-α, interferons, and iNOS. CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD200R1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

CD200R1 is an inhibitory receptor that acts as a key regulator by binding to the CD200/OX2 cell surface glycoprotein. Its regulatory role extends to limiting inflammation in response to specific stimuli by inhibiting pro-inflammatory molecules including TNF-α, interferons, and inducible nitric oxide synthase (iNOS). CD200R1 exhibits comparable affinity and kinetics when binding to the human herpesvirus 8 K14 viral CD200 homolog. The interaction between CD200 and CD200R1 is facilitated by their respective N-terminal Ig-like domains. In addition, CD200R1 binds to the human herpesvirus 8 vOX2 protein, further extending its multifunctional interactions in cellular processes.

Verified Bioactivity

Immobilized Biotinylated Human CD200 R1 at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Human CD200 with the EC50 of 19.7 ng/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-8*His
  • Accession

    Q8TD46-4 (A27-L266)

  • Gene ID
    131450  [NCBI]
  • Molecular Construction
    • N-term
    • CD200R1 (A27-L266)
      Accession # Q8TD46-4
    • 8*His-Avi
    • C-term
  • Protein Length

    Extracellular Domain

  • Conjugation

    Biotin

  • Synonyms

    CD200R1; CD200 Cell Surface Glycoprotein Receptor; Prev. MOX2R; MOX2 Receptor; CD200R; Cell Surface Glycoprotein Receptor CD200; OX2R; CD200R1 Protein; HCRTR2; CRTR2; Cell Surface Glycoprotein CD200 Receptor 1; CD200 Receptor 1; Cell Surface Glycoprotein

  • AA Sequence

    AAQPNNSLMLQTSKENHALASSSLCMDEKQITQNYSKVLAEVNTSWPVKMATNAVLCCPPIALRNLIIITWEIILRGQPSCTKAYKKETNETKETNCTDERITWVSRPDQNSDLQIRTVAITHDGYYRCIMVTPDGNFHRGYHLQVLVTPEVTLFQNRNRTAVCKAVAGKPAAHISWIPEGDCATKQEYWSNGTVTVKSTCHWEVHNVSTVTCHVSHLTGNKSLYIELLPVPGAKKSAKL

  • Predicted Molecular Mass

    29.7 kDa

  • Molecular Weight

    Approximately 52-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) Product Image