CD28 Protein, Human/Cynomolgus (Biotinylated, HEK293, His-Avi)

The CD28 protein is critical for T cell activation, enhancing proliferation, cytokine production, and survival. When linked to TCR/CD3 and CD40L, CD28 promotes the production of IL4 and IL10, thereby regulating immune responses. CD28 Protein, Human/Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus, human-derived CD28 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of CD28 Protein, Human/Cynomolgus (Biotinylated, HEK293, His-Avi) is 134 a.a., with molecular weight of 38-50 kDa.

CD28 protein plays a pivotal role in T-cell activation, promoting cell proliferation, cytokine production, and T-cell survival. Upon ligation with TCR/CD3 and CD40L costimulation, CD28 enhances the production of IL4 and IL10 in T-cells, contributing to immune response modulation. Additionally, isoform 3 of CD28 facilitates CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. The protein forms homodimers through disulfide linkages and interacts with various molecules, including DUSP14, CD80/B7-1, CD86/B7-2/B70, and GRB2. Isoform 3 specifically interacts with CD40LG, highlighting its multifaceted role in mediating immune responses and cellular signaling pathways.

1. Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD28 at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CD28 Antibody hFc with the EC₅₀ ≤ 20.2 ng/mL determined by ELISA.
2. Immobilized Anti-CD28 Antibody, hFc Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC₅₀ ≤ 24.4 ng/mL determined by ELISA.

Cynomolgus; Human

HEK293

N-His;N-Avi

P10747 (N19-P152)

940  [NCBI]

38-50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.