CD39 Protein, Human (Q96A, N99A, E143A, R147E, HEK293, His)

The CD39 protein is an important regulator in the nervous system, coordinating purinergic neurotransmission by hydrolyzing ATP and other nucleotides. Its enzymatic ability can hydrolyze platelet-activated ADP into AMP, thereby effectively preventing platelet aggregation. CD39 Protein, Human (Q96A, N99A, E143A, R147E, HEK293, His) is the recombinant human-derived CD39 protein, expressed by HEK293, with C-His labeled tag.

In the nervous system, the CD39 protein serves as a key regulator of purinergic neurotransmission by hydrolyzing ATP and other nucleotides. Its enzymatic activity extends to the prevention of platelet aggregation, as it efficiently hydrolyzes platelet-activating ADP to AMP. Notably, CD39 exhibits equal proficiency in the hydrolysis of both ATP and ADP, emphasizing its versatile role in modulating purinergic signaling pathways and contributing to the intricate regulation of neurotransmission and platelet function.

Human

HEK293

C-His

P49961 (T38-V478, Q96A, N99A, E143A, R147E)

953

Extracellular Domain

TQNKALPENVKYGIVLDAGSSHTSLYIYKWPAEKENDTGVVHQVEECRVKGPGISKFVAKVAEIGIYLTDCMERAREVIPRSQHQETPVYLGATAGMRLLRMESEALADEVLDVVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVPYETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYTHSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTPCTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFLPPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTSYAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGWTLGYMLNLTNMIPAEQPLSTPLSHSTYV

Approximately 60-70 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as 0.22 μm filtered solution of 20 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.